Disordered expression of the homing receptor glycans on helper memory T cells in patients with severe bronchial asthma

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790774
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: SAKUMA Keiichiro 愛知県がんセンター(研究所), 分子病態学部, 主任研究員 (90402891)
• Project Period (FY): 2010 – 2011
• Keywords: 閉塞性肺疾患 / 喘息 / ホーミング / メモリーT細胞 / 糖鎖

Research Abstract

1.The expression levels of the sulfated homing-receptor glycan on helper T(Th) cells were higher in patients with bronchial asthma or atopic dermatitis than those in normal controls. These levels were significantly correlated with frequency of exacerbation of atopic dermatitis, while the expression levels of non-sulfated Th-trafficking glycan were correlated with the disease severity at the time when the blood sample was collected. Statistic analysis suggested potential utility of the sulfated homing-receptor glycan for discriminating patients with highly recurrent atopic dermatitis.
2.We have reported the mechanism of the transcriptional regulation of a sulfotransferase gene HEC-GlcNAc6ST. We have also revealed the involvement of HEC-GlcNAc6ST in the synthesis of a sulfated cell-adhesion glycan in helper T cells.

Research Products

• [Journal Article] Colonic epithelial cells express specific ligands for mucosal macrophage immunosuppressive receptors siglec-7 and-9 (2012)
  • Author(s): Miyazaki K, Sakuma K, Kawamura Y, Izawa M, Ohmori K, Mitsuki M, Yamaji T, Hashimoto Y, Suzuki A, Saito Y, Dohi T, Kannagi R.
  • Journal Title: J Immunol Volume: 188(9) Pages: 4690-700
  • DOI: DOI:10.4049/jimmunol.1100605
  • Peer Reviewed
• [Journal Article] Induction of 6-sulfated glycans with cell adhesion activity via T-bet and GATA-3 in human helper T cells (2012)
  • Author(s): Sakuma K, Chen GY, Aoki M, Kannagi R.
  • Journal Title: Biochim Biophys Acta Volume: 1820 Pages: 841-848
  • DOI: DOI:10.1016/j.bbagen.2012.03.005
  • Peer Reviewed
• [Journal Article] Transcription factors c-MYC and CDX2 mediate E-selectin ligand expression in colon cancer cells undergoing EGF/bFGF-induced epithelial-mesenchymal transition (2012)
  • Author(s): Sakuma K, Aoki M, Kannagi R.
  • Journal Title: Proc Natl Acad Sci USA Volume: 109(20) Pages: 7776-7781
  • DOI: DOI:10.1073/pnas.1111135109
  • Peer Reviewed
• [Journal Article] Sialylated and sulfated carbohydrate ligands for selectins and siglecsinvolvement in traffic and homing of human memory T and B lymphocytes (2011)
  • Author(s): Kannagi R, Ohmori K, Chen GY, Miyazaki K, Izawa M, Sakuma K.
  • Journal Title: Adv Exp Med Biol Volume: 705 Pages: 549-569
  • DOI: DOI:10.1007/978-1-4419-7877-6_29
  • Peer Reviewed
• [Presentation] Th2-specific homing ligands involved in recurrent atopic dermatitis exacerbations. (2011)
  • Author(s): 佐久間圭一朗、古橋卓也、近藤幸子、矢部宇一郎、大森勝之、森田明理、神奈木玲児
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 千葉
  • Year and Date: 20111127-29
• [Presentation] EGF and bFGF induce EMT and E-selectin ligandglycan expression on colorectal cancer cells (2011)
  • Author(s): 佐久間圭一朗、青木正博、神奈木玲児
  • Organizer: 第70回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 20111003-05
• [Remarks]
  • URL: http://www.pref.aichi.jp/cancer-center/ri/01bumon/07bunshi_byotai/index.html