2011 Fiscal Year Final Research Report

Suppression of parathyroid hormone production in secondary hyperparathyroidism by RNA interference

Research Project

Project/Area Number	22790807
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Kidney internal medicine
Research Institution	Tokai University
Principal Investigator	KANAI Genta 東海大学, 医学部, 助教 (00535221)
Project Period (FY)	2010 – 2011
Keywords	人工透析学
Research Abstract	Secondary hyperparathyroidism(2HPT) is one of the most important complications of chronic kidney disease. Vitamin D3 receptor levels are decreased in the parathyroid glands of dialysis patients and animal models of renal failure. Recent research has shown that the functionality protein can be adjusted with the gene transfer to the parathyroid. We examined the parathyroid hormone(PTH) suppression by the RNA interference using rat 2HPT model. Serum PTH increased to a high level at the posterior when four weeks passed. Afterwards, high density anti-PTH siRNA was injected to rat's parathyroid glands. It was shown that serum PTH concentration was suppressed up to about 60%. The suppression effect was shown the peak on 1-2 weeks, and, then, maintained limitedly. In the present study, it was suggested that using the atelocollagen that was non-viruses drug delivery system contribute to the extension of effectiveness.

(2 results)