The role of secretory leukoprotease inhibitor(SLPI) in cerebral ischemia

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790827
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurology
• Research Institution: Keio University
• Principal Investigator: ABE Takato 慶應義塾大学, 医学部, 講師(非常勤) (30365233)
• Project Period (FY): 2010 – 2011
• Keywords: 脳虚血 / アストロサイト / 分泌型白血球ペプチダーゼ阻害物質

Research Abstract

First, we investigated glucose metabolism in astroglia during hypoxia. Hypoxia without glucoprivation induced pentose phosphate pathway(PPP) activation in astroglia. PPP activation by hypoxia was accompanied by Nrf2 translocation to the nucleus but not by Bip expression in the ER. Next, we investigated the effects of secretory leukoprotease inhibitor(SLPI) on the cell damage during ischemic-reperfusion injury. Alamar Blue reduction increased by SLPI during oxygen glucose deprivation in neurons, while SLPI did not affect astroglial cell viability. The effects of SLPI in cerebral ischemia might be different between astrocytes and neurons.

Research Products

• [Journal Article] Effects of fluctuating glucose concentrations on oxidative metabolism of glucose in cultured neurons and astroglia (2012)
  • Author(s): Takahashi S, Abe T, Izawa Y, Suzuki Y
  • Journal Title: Journal of Diabetes Mellitus Volume: 2 Pages: 19-26
  • Peer Reviewed
• [Presentation] Necessity of glucose for the activation of pentose phosphate pathway in astroglia under hypoxic conditions (2011)
  • Author(s): Takahashi S, Izawa Y, Abe T, Suzuki N
  • Organizer: Neuroscience Meeting 2011
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2011-11-12
• [Presentation] PO2変化に伴う培養アストログリアのペントースリン酸経路活性調節と脳虚血再灌流障害に対する神経保護機構 (2011)
  • Author(s): 伊澤良兼、高橋愼一、安部貴人、鈴木則宏
  • Organizer: 第36回脳卒中学会
  • Place of Presentation: 京都府
  • Year and Date: 2011-07-30
• [Presentation] マウス中大脳動脈・永久閉塞モデルと虚血再灌流モデルのin vivo脳微小循環動態の比較 (2010)
  • Author(s): 冨田裕、安部貴人、畝川美悠紀、鳥海春樹、正本和人、菅野巌、鈴木則宏
  • Organizer: 第22回日本脳循環代謝学会総会
  • Place of Presentation: 大阪府
  • Year and Date: 2010-11-27
• [Presentation] In vivo visualization of mouse cerebral microcirculation during middle cerebral artery occlusion infuced by the suture method (2010)
  • Author(s): Tomita Y, Abe T, Unekawa M, Toriumi H, Masamoto K, Kanno I, Suzuki N
  • Organizer: 7th World Stroke Congress
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2010-10-14
• [Presentation] Long-term in vivo investigation of mouse cerebral microcirculation after middle cerebral artery ischemia-reperfusion induced by the suture method (2010)
  • Author(s): Tomita Y, Abe T, Unekawa M, Toriumi H, Masamoto K, Kanno I, Suzuki N
  • Organizer: 9th World congress for microcirculation
  • Place of Presentation: Paris, France
  • Year and Date: 2010-09-28