The role of mitochondrial dysfunction and mitophagy in the cellular model of familial Parkinson's disease.

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790841
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurology
• Research Institution: 防衛大学校
• Principal Investigator: AMO Taku 防衛大学校, 応用科学群, 助教 (40453922)
• Project Period (FY): 2010 – 2011
• Keywords: パーキンソン病 / ミトコンドリア

Research Abstract

In PINK1-deficient mouse embryonic fibroblasts(MEFs), mitochondrial membrane potential and cellular ATP levels were decreased compared with those in littermate wild-type MEFs. However, mitochondrial proton leak, which reduces membrane potential in the absence of ATP synthesis, was not altered by loss of PINK1.Instead, activity of the respiratory chain, which produces the membrane potential by oxidizing substrates using oxygen, declined.

Research Products

• [Journal Article] Caloric restriction primes mitochondria for ischemic stress by deacetylating specific mitochondrial proteins of the electron transport chain (2011)
  • Author(s): Shinmura K, Tamaki K, Sano M, Nakashima-Kamimura N, Wolf AM, Amo T, Ohta S, Katsumata Y, Fukuda K, Ishiwata K, Suematsu M, Adachi T.
  • Journal Title: Circulation Research Volume: 109 Pages: 396-406
  • DOI: DOI:10.1161/CIRCRESAHA.111.243097
  • Peer Reviewed
• [Journal Article] Meat-type chickens have a higher efficiency of mitochondrial oxidative phosphorylation than laying-type chickens (2011)
  • Author(s): Toyomizu M, Kikusato M, Kawabata Y, Azad MA, Inui E, Amo T.
  • Journal Title: Comparative Biochemistry and Physiology Part A : Molecular and Integrative Physiology Volume: 159 Pages: 75-81
  • DOI: DOI:10.1016/j.cbpa.2011.01.020
  • Peer Reviewed
• [Journal Article] Mitochondrial DNA alterations in colorectal cancer cell lines (2011)
  • Author(s): Chihara N, Amo T, Tokunaga A, Yuzuriha R, Wolf AM, Asoh S, Suzuki H, Uchida E, Ohta S.
  • Journal Title: Journal of Nippon Medical School Volume: 78 Pages: 13-21
  • DOI: DOI:10.1272/jnms.78.13
  • Peer Reviewed
• [Journal Article] Mitochondrial membrane potential decrease caused by loss of PINK1 is not due to proton leak, but to respiratory chain defects (2011)
  • Author(s): Amo T, Sato S, Saiki S, Wolf AM, Toyomizu M, Gautier CA, Shen J, Ohta S, Hattori N.
  • Journal Title: Neurobiology of Disease Volume: 41 Pages: 111-118
  • DOI: DOI:10.1016/j.nbd.2010.08.027
  • Peer Reviewed
• [Journal Article] Time course of ROS production in skeletal musclemitochondria from chronic heat-exposed broiler chicken (2010)
  • Author(s): Azad MA, Kikusato M, Sudo S, Amo T, Toyomizu M.
  • Journal Title: Comparative Biochemistry and Physiology Part A : Molecular and Integrative Physiology Volume: 157 Pages: 266-271
  • DOI: DOI:10.1016/j.cbpa.2010.07.011
  • Peer Reviewed
• [Journal Article] Application of modular kinetic analysis to mitochondrial oxidative phosphorylation in skeletal muscle of birds exposed to acute heat stress (2010)
  • Author(s): Kikusato M, Ramsey JJ, Amo T, Toyomizu M.
  • Journal Title: FEBS Letters Volume: 584 Pages: 3143-3148
  • DOI: DOI:10.1016/j.febslet.2010.05.057
  • Peer Reviewed
• [Presentation] Modular kinetic analysis as a powerful tool for investigation of mitochondrial bioenergetics (2011)
  • Author(s): Amo T, Sato S, Saiki S, Kikusato M, Kawabata Y, Toyomizu M, Gautier C, Shen J, Ohta S, Hattori N, Brand MD
  • Organizer: 第5回アジア酸化ストレス学会年会・第8回アジアミトコンドリア学会年会・第11回日本ミトコンドリア学会年会合同大会
  • Place of Presentation: 鹿児島市民文化ホール(鹿児島県)
  • Year and Date: 2011-09-02
• [Presentation] PINK1ノックアウトMEFのミトコンドリア機能解析 (2011)
  • Author(s): 佐藤栄人、天羽拓、斉木臣二、Wolf AM、豊水正昭、Gautier CA、Shen J、太田成男、服部信孝
  • Organizer: 52回日本神経学会学術大会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-05-18
• [Presentation] Mitochondrial membrane potential decrease caused by loss of PINK1 is not due to proton leak, but to respiratory chain defects (2010)
  • Author(s): 天羽拓、佐藤栄人、斉木臣二、Wolf AM、豊水正昭、Gautier CA、Shen J、太田成男、服部信孝
  • Organizer: 33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 20101207-08
• [Presentation] Mitochondrial energetics and ROS production in chicken skeletal muscle : application of modular kinetic analysis (2010)
  • Author(s): Toyomizu M, Kikusato M, Amo T, Azad MAK
  • Organizer: 3rd EAAP International Symposium of Energy and protein metabolism and nutrition
  • Place of Presentation: Parma, Italy
  • Year and Date: 20100906-10
• [Presentation] Mitochondrial membrane potential decrease caused by loss of PINK1 is not due to proton leak, but to respiratory chain defects (2010)
  • Author(s): Amo T, Sato S, Saiki S, Wolf AM, Toyomizu M, Gautier CA, Shen J, Ohta S, Hattori N.
  • Organizer: The 7th Conference of Asian Society for Mitochondrial Research and Medicine and the 10th Conference of Japanese Society of Mitochondrial Research and Medicine
  • Place of Presentation: Fukuoka International Congress Center
  • Year and Date: 2010-12-16
• [Presentation] 肉用鶏のミトコンドリア酸化的リン酸化効率は産卵鶏より良い (2010)
  • Author(s): 豊水正昭,喜久里基,川端雄亮, Azad MAK,乾恵理子,天羽拓
  • Organizer: 日本家禽学会2010年度秋季大会
  • Place of Presentation: 信州大学農学部(長野県)
  • Year and Date: 2010-09-15
• [Presentation] Caloric restriction primes mitochondria for ischemic stress by deacetylating mitochondrial proteins (2010)
  • Author(s): Shinmura K, Yae K, Tamaki K, Sano M, Katsumata Y, Ishiwata K, Adachi T, Kamimura N, Wolf AM, Amo T, Ohta S.
  • Organizer: 第1回Molecular Cardiovascular Conference II
  • Place of Presentation: キロロ(北海道)
  • Year and Date: 2010-09-04
• [Presentation] Dose-dependent effects of resveratrol on mitochondrial function and myocardial ischemia/reperfusion injury. (2010)
  • Author(s): Shinmura K, Tamaki K, Sano M, Kamimura N, Wolf AM, Amo T, Ohta S.
  • Organizer: 20th World congress of the International Society for Heart Research
  • Place of Presentation: Kyoto International Conference Center(京都府)
  • Year and Date: 2010-05-14