Investigation of the role of insulin signaling molecules on development of hepatocytes into hepatocytes with fatty changes

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790847
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Metabolomics
• Research Institution: The University of Tokyo
• Principal Investigator: FUJISHIRO Midori 東京大学, 医学部附属病院, 助教 (50420211)
• Project Period (FY): 2010 – 2011
• Keywords: エネルギー / 糖質代謝異常

Research Abstract

We have found that chronic hyperglycemia induced marked increase of insulin receptor expression in the liver cells. I investigated if the mechanism of this change involved in a vicious cycle of hepatic insulin resistance. First, I analyzed protein expression levels of transcription factors predicted to bind with insulin receptor promoter regions and their cofactors of the various insulin acting organs with chronic high glucose stimulation, but could not identify the protein that changed its expression levels during such stimulation. Now I am further analyzing the changes during chronically high glucose stimulation with hyperinsulinemia

Research Products

• [Journal Article] Tomoichiro Asano、Xanthine oxidoreductase is involved in macrophage foam cell formation and atherosclerosis development (2012)
  • Author(s): Akifumi Kushiyama, Hirofumi Okubo, Hideyuki Sakoda, Takako Kikuchi, Midori Fujishiro, Hirokazu Sato, Sakura Kushiyama, Misaki Iwashita, Fusanori Nishimura, Toshiaki Fukushima, Yusuke Nakatsu, Hideaki Kamata, Shoji Kawazu, Yukihito Higashi, Hiroki Kurihara
  • Journal Title: Arterioscler Thromb Vasc Biol Volume: 32(2) Pages: 291-298
  • DOI: DOI:10.1161/ATVBAHA.111.234559
  • Peer Reviewed
• [Journal Article] Valsartan, independently of AT1 receptor or PPARγ, suppresses LPS-induced macrophage activation and improves insulin resistance in cocultured adipocytes (2012)
  • Author(s): Misaki Iwashita, Hideyuki Sakoda, Akifumi Kushiyama, Midori Fujishiro, Haruya Ohno, Yusuke Nakatsu, ToshiakiFukushima, Sonoko Kumamoto, Yoshihiro Tsuchiya, Takako Kikuchi, Hiroki Kurihara, Hiroshi Akazawa, Issei Komuro, Hideaki Kamata, Fusanori Nishimura
  • Journal Title: Am J Physiol Endocrinol Metab Volume: 302(3) Pages: E286-E296
  • DOI: DOI:10.1152/ajpendo.00324.2011
  • Peer Reviewed
• [Journal Article] Peptidyl-prolyl cis/trans isomerase NIMA-interacting 1 associates with insulin receptor substrate-1 and enhances insulin actions and adipogenesis (2011)
  • Author(s): Yusuke Nakatsu, Hideyuki Sakoda, Akifumi Kushiyama, Jun Zhang, Hiraku Ono, Midori Fujishiro, Takako Kikuchi, Toshiaki Fukushima, Masayasu Yoneda, Haruya Ohno, Nanao Horike, Machi Kanna, Yoshihiro Tsuchiya, Hideaki Kamata, Fusanori Nishimura, Toshiaki Isobe, Takehide Ogihara, Hideki Katagiri, Yoshitomo Oka, Shin-ichiro Takahashi, Hiroki Kurihara Takafumi Uchida and Tomoichiro Asano
  • Journal Title: J Biol Chem Volume: 286(23) Pages: 20812-2022
  • DOI: DOI:10.1074/jbc.M110.206904
  • Peer Reviewed
• [Journal Article] 4F2hc stabilizes GLUT1 protein and increases glucose transport activity (2011)
  • Author(s): Haruya Ohno, Yusuke Nakatsu, Hideyuki Sakoda, Akifumi Kushiyama, Hiraku Ono, Midori Fujishiro, Yuichiro Otani, Hirofumi Okubo, Masayasu Yoneda, Toshiaki Fukushima, Yoshihiro Tsuchiya, Hideaki Kamata, Fusanori Nishimura, Hiroki Kurihara, Hideki Katagiri, Yoshitomo Oka and Tomoichiro Asano
  • Journal Title: Am J Physiol Cell Physiol Volume: 300(5) Pages: C1047-C1054
  • DOI: DOI:10.1152/ajpcell.00416.2010
  • Peer Reviewed
• [Journal Article] Tomoichiro Asanol、LST8levelcontrolsbasalp70 S6 kinase and Akt phosphorylations, and mTORC1 and mTORC2 negatively regulate each other by competing for association with LST8
  • Author(s): Takako Kikuchi, Jun Zhang, Hideyuki Sakoda, Yuko Koketsu, Midori Fujishiro, Akifumi Kushiyama, Yusuke Nakatsu, Hideaki Kamata, Ken Inoki, Shin-Ichiro Takahashi, Hiroki Kurihara, Hideki Katagiri, Yoshitomo Oka
  • Journal Title: Obesity Research & Clinical Practice
  • DOI: DOI:10.1016/j.orcp.2011.10.002
  • Peer Reviewed
• [Presentation] 長時間高グルコース刺激による肝細胞内インスリン受容体遺伝子発現調節機構の解明 (2010)
  • Author(s): 藤城緑
  • Organizer: 第53回日本糖尿病学会年次学術集会
  • Place of Presentation: 岡山市デジタルミュージアム(岡山県)
  • Year and Date: 2010-05-28