2011 Fiscal Year Final Research Report
Functional analysis of a diabetes-susceptibility gene KCNQ1 in pancreatic beta cells
| Project/Area Number |
22790876
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Metabolomics
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| Research Institution | Research Institute, International Medical Center of Japan |
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Principal Investigator |
UDAGAWA Haruhide 独立行政法人国立国際医療研究センター, 研究所・糖尿病研究センター・代謝疾患研究部, 研究員 (50533882)
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| Project Period (FY) |
2010 – 2011
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| Keywords | エネルギー / 糖質代謝異常 |
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| Research Abstract |
This research was performed in order to elucidate the functional significance in pancreatic beta cells of KCNQ1, which was recently established by genome-wide association studies(GWAS) as one of the most important genetic factors of type 2 diabetes mellitus in most ethnic groups including Japanese. First, the expression of Kcnq1 was confirmed both in rat pancreatic beta cell-line INS-1 and mouse islets, and the changes in expression or activity of Kcnq1 resulted in dysregulation of insulin secretion in INS-1 cells. Second, multiple novel transcripts were demonstrated from the genomic region of intron 15 of Kcnq1 encompassing disease-related SNPs. Finally, Kcnq1-suppressed INS-1 cells was exposed to palmitate as a model of gene-environment interaction, and no significant interaction was observed in our system.
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