2012 Fiscal Year Final Research Report
Analysis of wheat protein in sera from patients with wheat allergy using mass spectrometer
| Project/Area Number |
22790930
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | Shimane University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | 食物アレルギー |
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| Research Abstract |
The aims of this study were to identify wheat proteins in sera of patient with wheat dependent exercise-induced anaphylaxis (WDEIA) during provocation tests using iTRAQ-based analysis, and to evaluate usefulness of CAP-fluorescent enzyme-immunoassay (CAP-FEIA) using recombinant high molecular weight-glutein. In iTRAQ-based analysis, although wheat proteins were detected in patients’ sera, those wheat proteins were not involving with allergic reactions. When recombinant HMW-glutenin was immobilized to CAP-FEIA, it was confirmed that detection of specific IgE anaginst recombinant HMW-glutenin is useful for diagnosis of WDEIA when combined with the CAP-FEIA (recombinant omega-5 gliadin) test.
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