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2012 Fiscal Year Final Research Report

Analysis of wheat protein in sera from patients with wheat allergy using mass spectrometer

Research Project

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Project/Area Number 22790930
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field 膠原病・アレルギー・感染症内科学
Research InstitutionShimane University

Principal Investigator

TAKAHASHI Hitoshi  島根大学, 医学部, 助教 (10432618)

Project Period (FY) 2010 – 2012
Keywords食物アレルギー
Research Abstract

The aims of this study were to identify wheat proteins in sera of patient with wheat dependent exercise-induced anaphylaxis (WDEIA) during provocation tests using iTRAQ-based analysis, and to evaluate usefulness of CAP-fluorescent enzyme-immunoassay (CAP-FEIA) using recombinant high molecular weight-glutein. In iTRAQ-based analysis, although wheat proteins were detected in patients’ sera, those wheat proteins were not involving with allergic reactions. When recombinant HMW-glutenin was immobilized to CAP-FEIA, it was confirmed that detection of specific IgE anaginst recombinant HMW-glutenin is useful for diagnosis of WDEIA when combined with the CAP-FEIA (recombinant omega-5 gliadin) test.

  • Research Products

    (9 results)

All 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (4 results)

  • [Journal Article] Serum gliadin monitoring extracts patients with false negative results in challenge tests for the diagnosis of wheat-dependent exercise-induced anaphylaxis.2013

    • Author(s)
      Kohno K, Matsuo H, Takahashi H, Niihara H, Chinuki Y, Kaneko S, Honjoh T, Horikawa T, Mihara S, Morita E.
    • Journal Title

      Allergol Int.

      Volume: 62 Pages: 229-38

    • Peer Reviewed
  • [Journal Article] Higher allergenicity of high molecular weight hydrolysed wheat protein in cosmetics for percutaneous sensitization.2013

    • Author(s)
      Chinuki Y, Takahashi H, Dekio I, Kaneko S, Tokuda R, Nagao M, Fujisawa T, Morita E.
    • Journal Title

      Contact Dermatitis.

      Volume: 68 Pages: 86-93

    • Peer Reviewed
  • [Journal Article] CD203c expression-based basophil activation test for diagnosis of wheat-dependent exercise-induced anaphylaxis.2012

    • Author(s)
      Chinuki Y, Kaneko S, Dekio I, Takahashi H, Tokuda R, Nagao M, Fujisawa T, Morita E.
    • Journal Title

      J Allergy Clin Immunol.

      Volume: 129 Pages: 1404-6

    • Peer Reviewed
  • [Journal Article] Prevalence of wheat allergy in Japanese adults.2012

    • Author(s)
      Morita E, Chinuki Y, Takahashi H,Nabika T, Yamasaki M, Shiwaku K.
    • Journal Title

      Allergol Int.

      Volume: 61 Pages: 101-5

    • Peer Reviewed
  • [Journal Article] Recombinant high molecular weight-glutenin subunit-specificIgE detection is useful in identifying wheat-dependent exercise-induced anaphylaxis complementary to recombinant omega-5 gliadin-specific IgE test.2012

    • Author(s)
      Takahashi H, Matsuo H, Chinuki Y,Kohno K, Tanaka A, Maruyama N,Morita E.
    • Journal Title

      Clin Exp Allergy.

      Volume: 42 Pages: 1293-8

    • Peer Reviewed
  • [Presentation] リコンビナント小麦蛋白質特異 IgE 測定の有用性.2012

    • Author(s)
      千貫祐子、松尾裕彰、新原邦江、 高橋仁、田中昭、森田栄伸.
    • Organizer
      第42回日本皮膚アレルギー・接触皮膚炎学会総会学術大会
    • Place of Presentation
      軽井沢プリンスホテルウエスト(軽井沢).
    • Year and Date
      20120713-15
  • [Presentation] 食物アレルギーとセツキシマブアレルギー.2012

    • Author(s)
      高橋 仁、千貫祐子、森田栄伸.
    • Organizer
      第42回日本皮膚アレルギー・接触皮膚炎学会総会学術大会
    • Place of Presentation
      軽井沢プリンスホテルウエスト(軽井沢).
    • Year and Date
      20120713-15
  • [Presentation] 牛肉アレルギーの抗原の同定とセツキシマブとの交叉反応性.2012

    • Author(s)
      高橋 仁、千貫祐子、森田栄伸.
    • Organizer
      第24回日本アレルギー学会春季臨床大会
    • Place of Presentation
      大阪国際会議場(大阪市).
    • Year and Date
      20120511-13
  • [Presentation] 小麦水溶性アレルゲンの大腸菌における可溶化発現.2012

    • Author(s)
      松山梓、横大路智治、 高橋仁、森田栄伸、松尾裕彰.
    • Organizer
      日本皮膚科学会第124回山陰・第20回島根合同開催地方会
    • Place of Presentation
      ホテルサンルート米子(米子市).
    • Year and Date
      2012-07-22

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Published: 2014-08-29  

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