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2012 Fiscal Year Final Research Report

Clock genes analysis in human breast cancer

Research Project

  • PDF
Project/Area Number 22791243
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionHirosaki University

Principal Investigator

MOROHASHI Satoko  弘前大学, 医学(系)研究科(研究院), 助教 (90569592)

Project Period (FY) 2010 – 2012
Keywords時計遺伝子 / 乳癌
Research Abstract

Expression of clock genes, DEC1 and DEC2 were up-regulated in the MCF-7 human breast cancer cells by TNF-α in a concentration-dependent manner. TNF-α induced the apoptosis of MCF-7 cells. We also demonstrated that siRNA-mediated DEC2 knockdown, but not DEC1, increased the Bax expression, cleavage PARP and cleavage caspase-7 in MCF-7 cells. In conclusion, we speculated that DEC2 plays an important role in the regulation of both apoptosis and proliferation of the MCF-7 cells.
In human breast cancer MCF-7 cells, paclitaxel increased the expression of DEC1 and DEC2. However, DEC1 and DEC2 exhibited opposite effects in paclitaxel inducing apoptosis, That is DEC1 has pro-apoptotic, while DEC2 has anti-apoptotic function. Both the two transcription factors play important roles in regulating paclitaxel induced apoptosispathway.

  • Research Products

    (18 results)

All 2012 2011 2010

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (5 results) Book (2 results)

  • [Journal Article] The BHLH transcription factor DEC1 plays an important role in theepithelial-mesenchymal transition of pancreatic cancer.2012

    • Author(s)
      Wu Y, Sato F, Yamada T, Bhawal UK, Kawamoto T, Fujimoto K, Noshiro M, Seino H, Morohashi S, Hakamada K, Abiko Y, Kato Y, Kijima H
    • Journal Title

      Int J Oncol

      Volume: 41[Epub ahead of print] Pages: 1337-1346

    • DOI

      10.3892/ijo.2012.1559

    • Peer Reviewed
  • [Journal Article] The basichelix-loop-helix transcription factor DEC2 inhibits TGF-β-induced tumor progression in human pancreatic cancer BxPC-3 cells.2012

    • Author(s)
      Sato F, Kawamura H, Wu Y, Sato H, Jin D, Bhawal UK, Kawamoto T, Fujimoto K, Noshiro M, Seino H, Morohashi S, Kato Y, Kijima H
    • Journal Title

      Int J Mol Med

      Volume: 30(3) Pages: 495-501

    • DOI

      doi:10.3892/ijmm.2012.1037. Epub 2012 Jun19.

    • Peer Reviewed
  • [Journal Article] BHLH transcription factor DEC2 regulates pro-apoptotic factor Bim in human oral cancer HSC-3 cells.2012

    • Author(s)
      Wu Y, Sato F, Bhawal UK, Kawamoto T, Fujimoto K, Noshiro M, Seino H, Morohashi S, Kato Y, Kijima H
    • Journal Title

      Biomed Res

      Volume: 33(2) Pages: 75-82

    • Peer Reviewed
  • [Journal Article] Smad3 and Snail show circadian expression in human gingival fibroblasts, human mesenchymal stem cell, and in mouse liver.2012

    • Author(s)
      Sato F, Sato H, Jin D, Bhawal UK, Wu Y, Noshiro M, Kawamoto T, Fujimoto K, Seino H, Morohashi S, Kato Y, Kijima H
    • Journal Title

      BiochemBiophys Res Commun.

      Volume: 419(2) Pages: 441-6

    • DOI

      doi:10.1016/j.bbrc.2012.02.076.

    • Peer Reviewed
  • [Journal Article] Intimal sarcoma of the pulmonary artery--diagnostic challenge.2011

    • Author(s)
      Fukuda W, Morohashi S, Fukuda I
    • Journal Title

      Acta Cardiol

      Volume: 66(4) Pages: 539-41

    • Peer Reviewed
  • [Journal Article] Histopathological phenotypes of early gastric cancer and its background mucosa.2011

    • Author(s)
      Kudo Y, Morohashi S, Takasugi K, Tsutsumi S, Ogasawara H, Hanabata N, Yoshimura T, Sato F, Fukuda S, Kijima H
    • Journal Title

      Biomed Res

      Volume: 32(2) Pages: 127-34

    • Peer Reviewed
  • [Journal Article] PERIOD1 (PER1) has anti-apoptotic effects, and PER3 has pro-apoptotic effects during cisplatin (CDDP) treatment in human gingival cancer CA9-22 cells.2011

    • Author(s)
      Sato F, Wu Y, Bhawal UK, Liu Y, Imaizumi T, Morohashi S, Kato Y, Kijima H
    • Journal Title

      Eur J Cancer

      Volume: 47(11) Pages: 1747-58

    • DOI

      doi:10.1016/j.ejca.2011.02.025.

    • Peer Reviewed
  • [Journal Article] Basic helix-loop-helix transcription factors DEC1 and DEC2 regulate the paclitaxel-induced apoptotic pathway of MCF-7 human breast cancer cells.2011

    • Author(s)
      Wu Y, Sato F, Bhawal UK, Kawamoto T, Fujimoto K, Noshiro M, Morohashi S,Kato Y, Kijima H.
    • Journal Title

      Int J MolMed

      Volume: 27(4) Pages: 491-495

    • Peer Reviewed
  • [Journal Article] Anti-apoptotic effect of claudin-1 in tamoxifen-treated human breast cancer MCF-7 cells.2010

    • Author(s)
      Akasaka H, Sato F, Morohashi S, Wu Y, Liu Y, Kondo J, Odagiri H, Hakamada K, Kijima H
    • Journal Title

      BMC Cancer

      Volume: 10 Pages: 548

    • DOI

      doi:10.1186/1471-2407-10-548.

    • Peer Reviewed
  • [Journal Article] Vimentin expression of esophageal squamous cell carcinoma and its aggressive potential for lymph node metastasis.2010

    • Author(s)
      Jin H, Morohashi S, Sato F, Kudo Y, Akasaka H, Tsutsumi S, Ogasawara H, Miyamoto K, Wajima N, Kawasaki H, Hakamada K, Kijima H
    • Journal Title

      Biomed Res

      Volume: 31(2) Pages: 105-112

    • Peer Reviewed
  • [Journal Article] Anti-apoptotic effect of the basic helix-loop-helix (bHLH) transcription factor DEC2 in human breast cancer cells.2010

    • Author(s)
      Liu Y, Sato F, Kawamoto T, Fujimoto K, Morohashi S, Akasaka H, Kondo J, Wu Y, Noshiro M, Kato Y, Kijima H
    • Journal Title

      Genes Cells

      Volume: 15(4) Pages: 315-25

    • DOI

      doi:10.1111/j.1365-2443.

    • Peer Reviewed
  • [Presentation] 異型濾胞上皮の結節性増生を示したGraves病甲状腺の一例2011

    • Author(s)
      諸橋聡子
    • Place of Presentation
      東京
    • Year and Date
      2011-11-18
  • [Presentation] ヒト乳癌細胞株MCF-7におけるbHLH型転写因子DEC2のアポトーシス制御機構の解析2011

    • Author(s)
      諸橋聡子
    • Organizer
      日本乳癌学会
    • Place of Presentation
      仙台市
    • Year and Date
      2011-09-03
  • [Presentation] 肺動脈内膜肉腫の1剖検例2011

    • Author(s)
      諸橋聡子
    • Organizer
      日本病理学会
    • Place of Presentation
      横浜市
    • Year and Date
      2011-04-28
  • [Presentation] 診断が困難であった乳腺症型繊維腺腫の1例の病理学的検討2010

    • Author(s)
      諸橋聡子
    • Organizer
      臨床外科学会
    • Place of Presentation
      横浜市
    • Year and Date
      2010-11-21
  • [Presentation] 乳腺に発生した腺脂肪腫の1例2010

    • Author(s)
      諸橋聡子
    • Organizer
      日本乳癌学会
    • Place of Presentation
      札幌市
    • Year and Date
      2010-06-25
  • [Book] 腫瘍病理鑑別診断アトラス食道癌2012

    • Author(s)
      諸橋聡子
    • Total Pages
      59-66
    • Publisher
      文光堂
  • [Book] 腫瘍病理鑑別診断アトラス大腸癌2011

    • Author(s)
      諸橋聡子
    • Total Pages
      112-125
    • Publisher
      文光堂

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Published: 2014-08-29   Modified: 2023-03-16  

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