2012 Fiscal Year Final Research Report
Clock genes analysis in human breast cancer
| Project/Area Number |
22791243
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Hirosaki University |
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Principal Investigator |
MOROHASHI Satoko 弘前大学, 医学(系)研究科(研究院), 助教 (90569592)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 時計遺伝子 / 乳癌 |
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| Research Abstract |
Expression of clock genes, DEC1 and DEC2 were up-regulated in the MCF-7 human breast cancer cells by TNF-α in a concentration-dependent manner. TNF-α induced the apoptosis of MCF-7 cells. We also demonstrated that siRNA-mediated DEC2 knockdown, but not DEC1, increased the Bax expression, cleavage PARP and cleavage caspase-7 in MCF-7 cells. In conclusion, we speculated that DEC2 plays an important role in the regulation of both apoptosis and proliferation of the MCF-7 cells.
In human breast cancer MCF-7 cells, paclitaxel increased the expression of DEC1 and DEC2. However, DEC1 and DEC2 exhibited opposite effects in paclitaxel inducing apoptosis, That is DEC1 has pro-apoptotic, while DEC2 has anti-apoptotic function. Both the two transcription factors play important roles in regulating paclitaxel induced apoptosispathway.
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Research Products
(18 results)
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[Journal Article] The BHLH transcription factor DEC1 plays an important role in theepithelial-mesenchymal transition of pancreatic cancer.2012
Author(s)
Wu Y, Sato F, Yamada T, Bhawal UK, Kawamoto T, Fujimoto K, Noshiro M, Seino H, Morohashi S, Hakamada K, Abiko Y, Kato Y, Kijima H
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Journal Title
Int J Oncol
Volume: 41[Epub ahead of print]
Pages: 1337-1346
DOI
Peer Reviewed
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[Journal Article] The basichelix-loop-helix transcription factor DEC2 inhibits TGF-β-induced tumor progression in human pancreatic cancer BxPC-3 cells.2012
Author(s)
Sato F, Kawamura H, Wu Y, Sato H, Jin D, Bhawal UK, Kawamoto T, Fujimoto K, Noshiro M, Seino H, Morohashi S, Kato Y, Kijima H
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Journal Title
Int J Mol Med
Volume: 30(3)
Pages: 495-501
DOI
Peer Reviewed
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[Journal Article] Smad3 and Snail show circadian expression in human gingival fibroblasts, human mesenchymal stem cell, and in mouse liver.2012
Author(s)
Sato F, Sato H, Jin D, Bhawal UK, Wu Y, Noshiro M, Kawamoto T, Fujimoto K, Seino H, Morohashi S, Kato Y, Kijima H
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Journal Title
BiochemBiophys Res Commun.
Volume: 419(2)
Pages: 441-6
DOI
Peer Reviewed
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[Journal Article] Vimentin expression of esophageal squamous cell carcinoma and its aggressive potential for lymph node metastasis.2010
Author(s)
Jin H, Morohashi S, Sato F, Kudo Y, Akasaka H, Tsutsumi S, Ogasawara H, Miyamoto K, Wajima N, Kawasaki H, Hakamada K, Kijima H
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Journal Title
Biomed Res
Volume: 31(2)
Pages: 105-112
Peer Reviewed
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[Journal Article] Anti-apoptotic effect of the basic helix-loop-helix (bHLH) transcription factor DEC2 in human breast cancer cells.2010
Author(s)
Liu Y, Sato F, Kawamoto T, Fujimoto K, Morohashi S, Akasaka H, Kondo J, Wu Y, Noshiro M, Kato Y, Kijima H
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Journal Title
Genes Cells
Volume: 15(4)
Pages: 315-25
DOI
Peer Reviewed
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