Role of the FOXF2 gene in the cancer activated fibroblasts that support proliferation of colon cancer cells

2011 Fiscal Year Final Research Report

• Project/Area Number: 22791272
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: SULTANA Nishat 浜松医科大学, 医学部, リサーチアシスタント (80529503)
• Project Period (FY): 2010 – 2011
• Keywords: 癌付随線維芽細胞 / 大腸癌 / Foxf2遺伝子 / 増殖 / 腫瘍微小環境

Research Abstract

When colon cancer cells HT29 were transplanted with intestinal embryonic fibroblasts(IEFs) from the Foxf2-/-and wild-type mice, the efficiency and tumor size were highest in cotranslantation with the wild-type IEFs, higher in that with the Foxf2-/-IEFs compared to that without fibroblasts. I isolated RNas from IEFs from the Foxf2-/-and wild-type mice and applied to DNA microarray analysis. I found 23 genes were significantly higher in the wild-type compared to the Foxf2-/-mice. I chose one gene named Wnt5a and demonstrated that the Wnt5a-expressing fibroblasts increased the efficiency and tumor size of HT29 cell transplantation.

Research Products

• [Journal Article] Liver tumor formation by a mutant retinoblastoma protein in the transgenic mice is caused by an up-regulation of c-Myc target genes (2012)
  • Author(s): Wang B, Hikosaka K, Sultana N, Sharkar MTK, Noritake H, Kimura W, Wu Y-X, Kobayashi Y, Uezato T, Miura N
  • Journal Title: Biochem Biophys Res Commun Volume: 417 Pages: 601-606
  • Peer Reviewed
• [Journal Article] Expression of human factors CD81, claudin-1, scavenger receptor, and occludin in mouse hepatocytes does not confer susceptibility to HCV entry (2011)
  • Author(s): Hikosaka K, Noritake H, Kimura W, Sultana N, Skarkar MTK, Tagawa Y, Uezato T, Kobayashi Y, Wakita T, Miura N
  • Journal Title: Biomed Res Volume: 32 Pages: 143-150
  • Peer Reviewed
• [Journal Article] Irxl1 mutant mice show reduced tendon differentiation and no patterning defects in musculoskletal system development (2011)
  • Author(s): Kimura W, Machii M, Xue X-D, Sultana N, Hikosaka K, Sharkar MTK, Uezato T, Matsuda M, Koseki H, Miura N
  • Journal Title: Genesis Volume: 49 Pages: 2-9
  • Peer Reviewed
• [Presentation] Isl1-specific knockout of Sonic hedgehog results in craniofacial and cardiac defects (2011)
  • Author(s): Sharkar M, 他
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20111213-16
• [Presentation] Irx3 determines expression of fast and slow connexins in the ventricular conduction system of the mouse heart (2011)
  • Author(s): Sultana N, 他
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20111213-16
• [Presentation] Irx3遺伝子はマウス心臓心室刺激伝導系のコネキシンの発現を調節する (2011)
  • Author(s): 木村航, 他
  • Organizer: 第10回心臓血管発生研究会
  • Place of Presentation: 郡山
  • Year and Date: 20111007-08
• [Presentation] Irxl1 is required for tendon differentiation during mouse musculoskeletal system development (2011)
  • Author(s): Kimura W, et al.
  • Organizer: Joint Meeting of the German and Japanese Societies of Developmental Biology
  • Place of Presentation: Dresden Germany
  • Year and Date: 20110323-26
• [Presentation] Generation of the Tbx1-AmCyan transgenic mice and its application (2011)
  • Author(s): Kimura W, et al.
  • Organizer: Machanism of cardiac Growth, Death and Regeneration
  • Place of Presentation: Keystone CO.
  • Year and Date: 20110200
• [Presentation] Creation of a Tbx1-AmCyan1 transgenic mice and its application to characterize the Tbx1-expressing cells (2010)
  • Author(s): 木村航, 他
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20101200
• [Remarks]
  • URL: http://www2.hama-med.ac.jp/w1a/bio2/index-j.html