2011 Fiscal Year Final Research Report
Gene therapy for pathological vascular remodeling targeting bone marrow-derived smooth muscle progenitor cells
| Project/Area Number |
22791308
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Thoracic surgery
|
|---|
| Research Institution | Gifu University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Keywords | 遺伝子治療 / 血管リモデリング / 超音波 / ナノバブル / 平滑筋前駆細胞 |
|---|
| Research Abstract |
We investigated gene therapy for pathological vascular remodeling targeting bone marrow-derived smooth muscle progenitor cells. In vitro study, green fluorescent protein(GFP) gene was transferred to COS-7 cells using sonoporation and the expression was confirmed by Western blotting and fluorescence microscopy. In vivo study, GFP gene was transferred to rat carotid artery and the gene expression was confirmed by fluorescence microscopy. We will prepare nano-bubbles and evaluate the further effects using a functional gene.
|
