In vivo imaging of neuropathic pain using novel transgenic Bdkrb1-ffLuc mice

2011 Fiscal Year Final Research Report

• Project/Area Number: 22791400
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Keio University
• Principal Investigator: TSUJI Osahiko 慶應義塾大学, 医学部, 助教 (70424166)
• Project Period (FY): 2010 – 2011
• Keywords: ブラジキニンB1受容体 / ルシフェラーゼ発光 / 神経障害性疼痛モデル

Research Abstract

In this study, we generated a novel transgenic mouse expressing ffLuc, a novel reporter gene, which could express both fluorescent and luminescent proteins(a variant of YFP and Luc2), under the control of the Bdkrb1 promoter(Bdkrb1-ffLuc). In this novel mouse, we made well-known neuropathic pain models. First, we performed the Seltzer model, partial sciatic nerve ligation model. At 2 weeks post-injury, a robust luminescent signal was detected on the injured side, whereas a mild signal was also detected on the contralateral side. Furthermore, the luminescent signal was shifted from ipsilateral to contralateral side at 4 weeks post-injury.

Research Products

• [Presentation] In vivo imaging of neuropathic pain using novel transgenic mice (2011)
  • Author(s): Osahiko Tsuji, Tsunehiko Konomi, Kanehiro Fujiyoshi, Akimasa Yasuda, Morio Matsumoto, Kazuhiro Chiba, Yoshiaki Toyama, Hirotaka James Okano, Hideyuki Okano, Masaya Nakamura
  • Organizer: Neuroscience meeting 2011
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20111112-16
• [Presentation] ルシフェラーゼ発光による慢性疼痛のライブイメージング (2011)
  • Author(s): 辻収彦、許斐恒彦、藤吉兼浩、岡野James洋尚、安田明正、海苔聡、松本守雄、千葉一裕、岡野栄之、戸山芳昭、中村雅也
  • Organizer: 第26回日本整形外科学会基礎学術集会
  • Place of Presentation: 前橋
  • Year and Date: 20111020-21
• [Presentation] ルシフェラーゼ発光による神経因性疼痛のライブイメージング【第2報】 (2011)
  • Author(s): 辻収彦、許斐恒彦、藤吉兼浩、原央子、岡野James洋尚、安田明正、松本守雄、千葉一裕、岡野栄之、戸山芳昭、中村雅也
  • Organizer: 第40回日本脊椎脊髄病学会
  • Place of Presentation: 東京
  • Year and Date: 20110421-23
• [Presentation] In vivo imaging of neuropathic pain using novel transgenic mice (2010)
  • Author(s): Osahiko Tsuji, Hirotaka James Okano, Kanehiro Fujiyoshi, Yoshiaki Toyama, Hideyuki Okano, and Masaya Nakamura
  • Organizer: The 16^<th> Takeda Science Foundation Syposium on Bioscience
  • Place of Presentation: 東京
  • Year and Date: 20101201-02
• [Presentation] 新規遺伝子改変マウスを用いた神経因性疼痛のライブイメージング【第1報】 (2010)
  • Author(s): 辻収彦、岡野ジェイムス洋尚、神吉浩明、原央子、藤吉兼浩、許斐恒彦、安田明正、石井賢、松本守雄、千葉一裕、岡野栄之、戸山芳昭、中村雅也
  • Organizer: 第25回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 20101014-15
• [Presentation] 神経因性疼痛のライブイメージング【第1報】 (2010)
  • Author(s): 辻収彦、岡野James洋尚、神吉浩明、原央子、藤吉兼浩、安田明正、渡辺航太、辻崇、石井賢、松本守雄、千葉一裕、岡野栄之、戸山芳昭、中村雅也
  • Organizer: 第39回日本脊椎脊髄病学会
  • Place of Presentation: 高知
  • Year and Date: 20100421-23