2011 Fiscal Year Final Research Report
Identification of hydatidiform mole-related microRNAs and their clinical signification
| Project/Area Number |
22791535
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nagasaki University |
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Principal Investigator |
MIURA Shoko 長崎大学, 大学院・医歯薬学総合研究科, 客員研究員 (00404301)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 嚢胞化絨毛 / 分子マーカー / RNA / microRNA / マイクロアレイ |
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| Research Abstract |
The aim of this study was to identify the microRNA marker of the complete hydatidiform moles(CHMs) in plasma. By next generation sequencing and quantitative RT-PCR analysis, hsa-miR-520f, 520b, 520c-3p and 519b-3p were selected as candidate CHMs-associated microRNAs. The median plasma concentration of cell-free has-miR-520f in CHMs pregnancy was significantly higher than in normal pregnancy(27, 194 copies/ mL vs 13, 118 copies/ mL, Mann-Whitney U test, P=0. 002). In 15 cases of CHMs pregnancy, the plasma concentration of cell-free has-miR-520f after termination of pregnancy was significantly decreased than that before termination of pregnancy(wilcoxon signed rank test, P<0. 001). In conclusion, the hsa-miR-520f is CHMs-related microRNA and its plasma concentration may be possible molecular marker of complete hydatidiform mole.
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[Journal Article] Clinical application of fetal sex determination using cell-free fetal DNA in pregnant carriers of X-linked genetic disorders2011
Author(s)
Miura K, Higashijima A, Shimada T, Miura S, Yamasaki K, Abe S, Jo O, Kinoshita A, Yoshida A, Yoshimura S, Niikawa N, Yoshiura K, and Masuzaki H.
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Journal Title
Journal of Human Genetics
Volume: 56
Pages: 296-299
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[Journal Article] Epidemiology of human papillomavirus genotypes in pregnant Japanese women2011
Author(s)
Yamasaki K, Miura K, Shimada T, Miura S, Abe S, Murakami M, Sameshima T, Fujishita A, Kotera K, Kinoshita A, Yoshiura K, and Masuzaki H.
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Journal Title
Journal of Human Genetics
Volume: 56
Pages: 313-315
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[Journal Article] Pre-vaccination epidemiology of human papillomavirus infections in Japanese women with abnormal cytology2011
Author(s)
Yamasaki K, Miura K, Shimada T, Ikemoto R, Miura S, Murakami M, Sameshima T, Fujishita A, Kotera K, Kinoshita A, Yoshiura K, Masuzaki H.
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Journal Title
Journal of Obstetrics and Gynaecology Research
Volume: 37
Pages: 1666-1670
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