2011 Fiscal Year Final Research Report

Identification of carcinogenesis mechanism and strategy of novel treatment by microRNA analysis in head and neck cancer or gynecological cancer

Research Project

Project/Area Number	22791630
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Otorhinolaryngology
Research Institution	Keio University
Principal Investigator	UNO Kosuke 慶應義塾大学, 医学部, 助教 (20464828)
Project Period (FY)	2010 – 2011
Keywords	頭頸部癌 / マイクロRNA
Research Abstract	MicroRNAs(miRNAs), showing highly disease-specific expression patterns, are promising biomarkers and novel therapeutic targets. We first examined the expression of human miRNAs in laryngeal and pharyngeal tissues using microarrays, To confirm these findings, we next performed quantitative real-time PCR(qRT-PCR) on cancer specific miRNAs and these findings were consistent with those obtained from microarrays. Finally, to explore the physiological significances of laryngeal and pharyngeal cancer-associated miRNAs, we examined the effect of these miRNAs on cell growth and viability using head and neck cancer cell line. We couldn't carry out the study about gynecological cancer in this term.

(1 results)