2012 Fiscal Year Final Research Report
Establishment of a method to promote osteoblast differentiation using tryptophan derivatives and Dex, and elucidation of the mechanisms of them
| Project/Area Number |
22791778
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Morphological basic dentistry
|
|---|
| Research Institution | Nihon University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Keywords | デキサメサゾン / トリプトファン誘導体 / 骨芽細胞 |
|---|
| Research Abstract |
In this study, we investigated the effect of newly synthesized tryptophan derivatives and dexamethasone (Dex), a synthetic glucocorticoid, on osteoblast differentiation. The tryptophan derivatives and Dex enhanced terminal osteoblast differentiation, as indicated by mineralization. However, no inductive effect was observed on commitment of mesenchymal stem cells (MSC) into osteoblast differentiation was observed. Furthermore, it was suggested that a transcription factor, Osterix, played an important role in the mechanism by which tryptophan derivatives and dexamethasone induced terminal osteoblast differentiation. In addition, we demonstrated that these agents significantly induced mRNA expression levels of the osteogenic marker genes bone sialoprotein (BSP) and Alkaline Phosphatase (ALP) in MSCs co-cultured with osteoblasts.
|
-
-
-
-
[Journal Article] CD271/p75(NTR) Inhibits the Differentiation of Mesenchymal Stem Cells into Osteogenic, Adipogenic, Chondrogenic, and Myogenic Lineages.2011
Author(s)
Mikami Y, Ishii Y, Watanabe N, Shirakawa T, Suzuki S, Irie S, Isokawa K, Honda JM.
-
Journal Title
Stem Cells Dev.
Volume: 20
Pages: 901-913
DOI
Peer Reviewed
-
-
-
-
-
-
