2012 Fiscal Year Final Research Report
Analysis ofthe cell polarity in osteoclasts
| Project/Area Number |
22791792
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
MASUHARA Masaaki 鹿児島大学, 大学院・医歯学総合研究科, 講師 (70372901)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 破骨細胞 / 極性 / ケルセチン / エストロゲン受容体 |
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| Research Abstract |
Many of Rab-family GTPase and KIF-family motor proteins were expressed in osteoclast regardless of its differentiation stage. We analyzed the changes of localization of some molecules that are thought to have polarity using GFP fusion system and siRNA-knockdown system. All of the molecules we analyzed have no effects, and these results seem to show the redundancy of Rab-family proteins. 2, Quercetin, one of major flavonoids found in many fruits, inhibited the differentiation of osteoclast. The assay using specific agonist and antagonist showed that the effects of quercetin were principally via GPR30, GPCR-type estrogen receptor, and Akt phosphorylation pathway.
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[Journal Article] Receptor activator of NF-kB ligand-dependent expression of caveolin-1 in osteoclast precursors, and high dependency of osteoclastogenesis on exogenous lipoprotein2012
Author(s)
Hada N, Okayasu M, Ito J, Nakayachi M, Hayashida C, Kaneda T, Uchida N, Muramatsu T, Koike C, Masuhara M, Sato T, Hakeda Y
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Journal Title
Bone
Volume: 50(1)
Pages: 226-236
DOI
Peer Reviewed
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