Elucidation of mechanism of BMP signaling in bone invasion by oral squamous cell carcinoma cells

2011 Fiscal Year Final Research Report

• Project/Area Number: 22791795
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Saitama Medical University
• Principal Investigator: SHIN Masashi 埼玉医科大学, 医学部, 研究員 (70549261)
• Project Period (FY): 2010 – 2011
• Keywords: 口腔癌 / シグナル伝達 / 骨

Research Abstract

Cancer cells in the oral cavity frequently invade alveolar bone. Although bone invasion by oral carcinoma cells is a critical factor for determination of prognosis, molecular mechanisms are unclear because an animal model in vivo of this event has not been established yet. In the present study, we examined roles of BMP signaling in bone invasion by cancer cells in vivo and in vitro. Over-expression of a constitutively active BMP receptor in vitro induced morphological changes. A cell line stably expressing a constitutively active BMP receptor, but not an empty vector, showed bone invasion in vivo. These findings suggest that BMP signaling stimulates bone invasion by cancer cells.

Research Products

• [Journal Article] Selective inhibition of NF-κB suppresses bone invasion by oral squamouscell carcinoma in vivo (2012)
  • Author(s): Furuta H, Osawa K, Shin M, Ishikawa A, Matsuo K, Khan M, AokiK, Ohya K, Okamoto M, Tominaga K, TakahashiT, Nakanishi O, Jimi E
  • Journal Title: Int J Cancer Volume: (in press)
  • Peer Reviewed
• [Journal Article] Identification and functional analysis of Zranb 2 as a novel Smad-bindingprotein that suppresses BMP signaling (2012)
  • Author(s): Ohte S, Kokabu S, Iemura S, Sasanuma H, Yoneyama K, Shin M, Suzuki S, Fukuda T, Nakamura Y, Jimi E, NatsumeT, Katagiri T
  • Journal Title: J Cell Biochem Volume: 113 Pages: 808-814
  • Peer Reviewed
• [Journal Article] The inhibition of RANKL/RANK signalingby osteoprotegerin suppresses boneinvasion by oral squamous cell carcinoma cells (2011)
  • Author(s): Shin M, Matsuo K, Tada T, FukushimaH, Furuta H, Ozeki S, Kadowaki T, YamamotoK, Okamoto M, Jimi E.
  • Journal Title: Carcinogenesis Volume: 32 Pages: 1634-1640
  • Peer Reviewed
• [Journal Article] A novel mutation of ALK2, L196P, found in the most benign case of fibrodysplasia ossificans progressiva activates BMP-specific intracellular signaling equivalent to a typical mutation, R206H (2011)
  • Author(s): Ohte S, Shin M, Sasanuma H, Yoneyama K, Akita M, Ikebuchi K, Jimi E, Maruki Y, Matsuoka M, Namba A, Tomoda H, Okazaki Y, Ohtake A, Oda H, Owan I, Yoda T, Furuya H, Kamizono J, Kitoh H, Nakashima Y, Susami T, Haga N, Komori T, Katagiri T
  • Journal Title: Biochem Biophys Res Commun Volume: 407 Pages: 213-218
  • Peer Reviewed
• [Journal Article] Suppression of BMP-Smad signaling axis-induced osteoblastic differentiation by small C-terminaldomain phosphatase 1, a Smad phosphatase (2011)
  • Author(s): Kokabu S, Ohte S, Sasanuma H, Shin M, Yoneyama K, Murata E, Kanomata K, NojimaJ, Ono Y, Yoda T, Fukuda T, Katagiri T
  • Journal Title: Mol Endocrinol Volume: 25 Pages: 474-481
  • Peer Reviewed
• [Presentation] BMP初期応答遺伝子のIdファミリーにおけるBMP応答配列の同定 (2011)
  • Author(s): 進正史、古株彰一郎、自見英治郎、片桐岳信
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
  • Year and Date: 2011-10-01
• [Presentation] Identification of a Common BMP-responsive Element in the Id1, Id2 and Id3 Genes (2011)
  • Author(s): Masashi Shin, Satoshi Ohte, Katsumi Yoneyama, Hiroki Sasanuma, Shoichiro Kokabu, Toru Fukuda, Eijiro Jimi, Takenobu Katagiri
  • Organizer: 2011 ASBMR Annual Meeting
  • Place of Presentation: サンディエゴ(アメリカ)
  • Year and Date: 2011-09-17
• [Presentation] BMP signaling stimulates alveolar bone invasion by melanoma cells (2010)
  • Author(s): Masashi Shin, Hidefumi Fukushima, Hiroyuki Furuta, Kazuhiro Aoki, Masud Khan, Keiichi Ohya, Takenobu Katagiri, and Eijiro Jimi
  • Organizer: 16th International Conference of the INTERNATIONAL SOCIETY OF DIFFERENTIATION
  • Place of Presentation: 奈良
  • Year and Date: 2010-11-15
• [Presentation] 口腔癌細胞の顎骨浸潤におけるBMPシグナル活性化の役割 (2010)
  • Author(s): 進正史、福島秀文、古田弘之、青木和広、マスードカーン、大谷啓一、片桐岳信、自見英治郎
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-21
• [Presentation] 口腔扁平上皮癌の顎骨浸潤モデルにおけるNF-kBシグナルによる顎骨浸潤制御機構の解明 (2010)
  • Author(s): 古田弘之、進正史、福島秀文、自見英治郎
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-21
• [Remarks]
  • URL: http://www.saitama-med.ac.jp/genome/Div04_PPhysiol/index.html