Survival of salivary pleomorphic adenoma cells in hypoxic condition

2011 Fiscal Year Final Research Report

• Project/Area Number: 22791810
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Niigata University
• Principal Investigator: MARUYAMA Satoshi 新潟大学, 医歯学総合病院, 講師 (30397161)
• Project Period (FY): 2010 – 2011
• Keywords: 実験腫瘍学

Research Abstract

Salivary pleomorphic adenoma(PA) is histopathologically characaterized by its colorful stroma with myxoid appearances, which are poorly vascularized, and PA cells are supposed to be able to survive in hypoxic conditions. To understand the hypoxia-dependent manner of cellular proliferation, we determined both protein and gene expression levels of hypoxia-inducible factor 1α(HIF-1α), vascular endothelial growth factor(VEGF), and von Hippel-Lindau(vHL), which degrades HIF-1α. SM-AP cells, which I established from a human parotid pleomorphic adenoma, showed more enhanced levels of HIF-1α, and their VEGF protein levels were kept higher in hypoxic conditions than in aerobic ones. SM-AP cells showed lower expression levels for the vHL gene. Likewise, tumor tissue masses of transplanted SM-AP cells were lower in O_2 concentration than normal subcutanous tissue. The results indicate that pleomorphic adenoma cells can proliferate in the hypoxic condition in which HIF-1αprotein levels were maintained in higher levels because the degradation of HIF-1αwas inhibited due to the lower vHL protein levels. It is thus concluded that hypoxia is more beneficial for PA cell proliferation.

Research Products

• [Journal Article] Loss of keratin 13 in oral carcinoma in situ : a comparative study of protein and gene expression levels using paraffin sections (2012)
  • Author(s): Ida-Yonemochi H, Maruyama S, Kobayashi T, Yamazaki M, Cheng J, Saku T
  • Journal Title: Mod Pathol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Short telomeres in an oral precancerous lesion : Q-FISH analysis of leukoplakia (2012)
  • Author(s): Aida J, Kobayashi T, Saku T, Yamaguchi M, Shimomura N, Nakamura Kl, Ishikawa N, Maruyama S, Cheng J, Poon SS, Sawabe M, Arai T, Takubo K
  • Journal Title: J Oral Pathol Med Volume: 41(5) Pages: 372-378
  • Peer Reviewed
• [Journal Article] Enhanced expression of Podoplanin in oral carcinomas in situ and squamous cell carcinomas (2011)
  • Author(s): Funayama A, Cheng J, Maruyama S, Yamazaki M, Kobayashi T, Syafriadi M, Kundu S, Shingaki S, Saito C, Saku T
  • Journal Title: Pathobiology Volume: 78(3) Pages: 171-180
  • Peer Reviewed
• [Journal Article] Emergence of keratin 17 vs. loss of keratin 13 : Their reciprocal immunohistochemical profiles in oral carcinoma in situ (2011)
  • Author(s): Mikami T, Cheng J, Maruyama S, Kobayashi T, Funayama A, Yamazaki M, Adeola HA, Wu L, Shingaki S, Saito C, Saku T
  • Journal Title: Oral Oncol Volume: 47(6) Pages: 497-503
  • Peer Reviewed
• [Journal Article] Nuclear translocation of b-catenin synchronized with loss of E-cadherin in oral epithelial dysplasia with a characteristic two-phase appearance (2011)
  • Author(s): Alvarado CG, Maruyama S, Cheng J, Ida-Yonemochi H, Kobayashi T, Yamazaki M, Takagi R, Saku T
  • Journal Title: Histopathology Volume: 59(2) Pages: 283-291
  • Peer Reviewed
• [Journal Article] Oral cancer in Myanmar : a preliminary survey based on hospital-based cancer registries (2011)
  • Author(s): Oo HN, Myint YY, Maung CN, Oo PS, Cheng J, Maruyama S, Yamazaki M, Yagi M, Sawair FA, Saku T
  • Journal Title: J Oral Pathol Med Volume: 40(1) Pages: 20-6
  • Peer Reviewed
• [Journal Article] A case of desmoplastic ameloblastoma arising in the maxillary alveolus : the origin and time-couse changes in the early stage of tumor development observed on dental radiographs (2010)
  • Author(s): Katsura K, Maruyama S, Suzuki M, Saku T, Takagi R, Hayashi T
  • Journal Title: Dentomaxillofac Radiol Volume: 40(2) Pages: 126-129
  • Peer Reviewed
• [Journal Article] Combined immunohistochemistry for the differential diagnosis of cystic jaw lesions ; its practical use in surgical pathology (2010)
  • Author(s): Tsuneki M, Yamazaki M, Cheng J, Al-Eryani K, Maruyama S, Kobayashi T, Saku T
  • Journal Title: Histopathology Volume: 57(6) Pages: 806-813
  • Peer Reviewed
• [Journal Article] Acetic acid treatment for wrinkle-free oral mucosal epithelia in paraffin section preparation (2010)
  • Author(s): Ahsan MS, Maruyama S, Cheng J, Al-Eryani K, Yamazaki M, Hasegawa M, Tsuneki M, Saku T
  • Journal Title: Microsc Res Tech
  • Peer Reviewed
• [Presentation] ワークショップ1外科病理シリーズ「口腔癌取り扱い規約の課題」; WS1-2 :口腔癌早期病変(表在癌)の病理診断基準の確立へ向けて (2012)
  • Author(s): 丸山智
  • Organizer: 第30回日本口腔腫瘍学会総会・学術大会
  • Place of Presentation: さいたま市
  • Year and Date: 20120126-27
• [Presentation] 唾液腺多形性腺腫における低酸素応答性増殖機構 (2011)
  • Author(s): 丸山智,山崎学,程君,朔敬
  • Organizer: 第70回日本癌学会プログラム抄録集
  • Place of Presentation: 名古屋市
  • Year and Date: 20111003-05
• [Presentation] 口腔扁平上皮癌の浸潤性評価:パールカン免疫陽性間質の立体構築 (2011)
  • Author(s): 丸山智,島津徳人,工藤朝雄,青葉孝昭,朔敬
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 20110428-30
• [Presentation] 口腔扁平苔癬のリンパ球浸潤域とパールカン沈着:異型上皮との鑑別の試み (2010)
  • Author(s): 丸山智,山崎学,程君,朔敬
  • Organizer: 第21回日本臨床口腔病理学会総会
  • Place of Presentation: 大阪市
  • Year and Date: 20100731-0801
• [Presentation] 唾液腺多形性腺腫の低酸素環境における増殖機構: SM-AP細胞による検討 (2010)
  • Author(s): 丸山智,程君,山崎学,朔敬
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京都
  • Year and Date: 20100427-29