2012 Fiscal Year Final Research Report
Research for the regeneration of periodontal tissue using periodontal ligament-derived hemangioblasts-like cells
| Project/Area Number |
22791936
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | 再生歯学 |
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| Research Abstract |
In this study, we focused on the CDX2 gene such as a key gene that induces into hematopoietic stem cells. PDL cells over-expressing CDX2 gene were observed to differentiate into hematopoietic stem-like cells. In addition, we attempted to isolateof hematopoietic stem cells from mouse bone marrow. As a result, the hematopoietic stem cells of Sca-1, CD34, CD45-positive were obtained. These hematopoietic stem cells adhere to type IV collagen, but it does not adhere to type I collagen. Furthermore, hematopoietic stem cells proliferated vigorously by a feeder of mesenchymal stem cells. Elucidation of the induction of angiogenesis is expected by using these cells.
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