Quantitative proteomics using formalin-fixed paraffin-embedded tissues of oral cancer

2011 Fiscal Year Final Research Report

• Project/Area Number: 22791959
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: NEGISHI Ayako 東京医科歯科大学, 歯学部附属病院, 医員 (70549972)
• Project Period (FY): 2010 – 2011
• Keywords: プロテオーム / 口腔がん / バイオマーカー

Research Abstract

Neoadjuvant chemotherapy is used to treat oral cancer however chemo-resistance is a major obstacle. The ability to predict tumour response would allow chemotherapy administration to be directed towards patients who would most benefit, thus maximising treatment efficacy. This study aimed to identify protein biomarkers associated with response to neoadjuvant chemotherapy using formalin-fixed paraffin-embedded(FFPE) tissue blocks of oral squamous cell carcinoma samples and analyzed by 2-dimensional image-converted analysis of liquid chromatography and mass spectrometry(2DICAL), a label-free quantitative proteomics method.