2011 Fiscal Year Final Research Report
Visualization of molecular mechanisms of amyloid fibril formation
| Project/Area Number |
22810014
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | Osaka University |
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Principal Investigator |
YAGI Hisashi 大阪大学, 蛋白質研究所, 助教 (10432494)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 全反射蛍光顕微鏡 / アミロイド線維 / アルコール / HFIP / リアルタイム観察 / ヒトIslet amyloid polypeptide |
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| Research Abstract |
Understanding the molecular mechanisms of amyloid formation is important to prevent various amyloidosis. In this study, to elucidate the molecular mechanisms of amyloid fibril formation, we visualized the reaction of amyloid fibril growth by total internal reflection fluorescence microscopy. Using this method, we succeeded in distinguishing between the amyloid fibrils and amorphous aggregates. Then, we focused on amyloid fibril growth. We added Alexa-532 labeled peptides to preformed fibrils and then observed the fibril growth. Our results suggested that the growth of fibrils occurred in not only the terminal of fibrils but also the side surface of fibrils.
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