2022 Fiscal Year Research-status Report
Novel strategies to expand the scope of biological nanopores for sensing biomolecules
| Project/Area Number |
22K05330
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2027-03-31
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| Keywords | nanopore |
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| Outline of Annual Research Achievements |
Most of the expected research goals have been achieved. Particularly, designed proteins have been expressed and purified in small scales. Larger scale production is in progress. The chemical syntheses have been repeated. The purity of the chemicals is being further improved. The establishment of a UPLC-MS system is delayed but it is currently significantly improving the purity and characterisation of the chemicals. On the other hand, the delivery of the AKTAgo FPLC system was much delayed due to the devaluation of the Japanese Yen and difficulties in international delivery during the COVID pandemic. However, it has been set up and the purity of the proteins is being greatly improved. The preparation of novel nanopores are currently in progress. The establishments of the above-mentioned instruments together with a Benchtop NMR system will accelerate the progress of the project greatly.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
The delayed deliveries of essential instruments caused some slight delays in the progress of the project. However, the successful establishments of these devices will greatly accelerate the progress of the project from now on.
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| Strategy for Future Research Activity |
The project is expected to progress smoothly from now on.
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| Causes of Carryover |
It was not used because the remaining amount was not sufficient for the purchase of any useful consumables.
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