2022 Fiscal Year Research-status Report
Cardiac electrophysiology evaluation system for improved drug assessment
| Project/Area Number |
22K12801
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| Research Institution | Osaka University |
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Principal Investigator |
李 俊君 大阪大学, 大学院医学系研究科, 特任助教 (10723786)
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| Co-Investigator(Kenkyū-buntansha) |
劉 莉 大阪大学, 大学院医学系研究科, 特任准教授 (50380093)
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| Project Period (FY) |
2022-04-01 – 2025-03-31
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| Keywords | Pluripotent stem cells / Cardiomyocytes / Maturation / Drug assessment |
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| Outline of Annual Research Achievements |
We planned to reproduce the traveling wave on 2D plate and optimize the best condition for obtain matured hiPSC derived cardiomyocytes, to compare the traveling wave matured cardiomyocytes with adult cells. The achievements in the year include: 1. The traveling wave has been generated in cardiac tissue cultured on both plate and nanofiber sheets. The electrical signal of cardiac tissue could be recorded by using MEA. 2.The maturity of traveling wave matured cardiomyocytes has been confirmed by using staining, TEM, RNAsequencing and other methods. Their maturation level has been compared with those from human adult. 3. We published 2 papers and gave an oral presentation in 87th Annual Scientific Meeting of the Japanese Circulation Society, and obtained the young investigator award.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We have accomplished the objective of the first year rather smoothly. Now we have already been working according to second year plan.
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| Strategy for Future Research Activity |
In this year, we will focus on integrating the traveling wave matured cardiac tissue on a multi electrode array (MEA) system. The drug test will also be performed on the matured tissue with MEA system. The tissue culture method and the drug test condition will be optimized. The data collected during experiment will be summarized to prepare papers.
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[Presentation] Human iPSC-derived closed-loop cardiac tissue for improved drug assessment2023
Author(s)
Ying Hua, Junjun Li, Xiang Qu, Jingbo Zhang, Masako Ishida, Noriko Yoshida, Hayato Miyoshi, Mikio Shiba3, Shuichiro Higo4,5, Nagako Sougawa1, Maki Takeda1, Takuji Kawamura1, Ryohei Matsuura, Toshihiko Toyofuku, Yoshiki Sawa, Li Liu and Shigeru Miyagawa
Organizer
The 87th Annual Scientific Meeting of the Japanese Circulation Society (JCS2023)
Invited
