2023 Fiscal Year Final Research Report
On-chip reaction and processing process for precise preparation of functionalized cell-laden microgel particles
| Project/Area Number |
22K14547
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 27040:Biofunction and bioprocess engineering-related
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Keywords | マイクロ流体デバイス / ゲル粒子 / 反応・処理プロセス |
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| Outline of Final Research Achievements |
In this study, we propose a novel microfluidic technique for the continuous production of functionalized microgel particles containing a uniform number of cells by guiding target cell-encapsulated droplets across multiple laminar streams (e.g., reaction and processing solutions) formed in micropillar arrays. Using the constructed microfluidic system, we demonstrated the continuous production of cell-laden microgels without empty droplets by guiding only the cell-encapsulated droplets across the multiple laminar streams.
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| Free Research Field |
マイクロ・ナノ流体工学
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| Academic Significance and Societal Importance of the Research Achievements |
細胞包埋ゲル粒子を用いた細胞の解析は，基礎研究や再生医療用途など様々な分野への応用が近年注目されているが，細胞周囲の微小空間が精密に制御され，さらには環境応答性等の機能が付与された，機能性細胞包埋ゲル粒子の作製プロセス技術は未確立である．対して，機能性細胞包埋ゲル粒子をシームレスに精密調製可能なプロセス技術の確立を目指す本研究は，医学・生化学などの基礎研究，再生医療への応用など，新しい分野の開拓・発展を支える基盤技術になり得ると考えられる．
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