2022 Fiscal Year Research-status Report
Spatiotemporal metabolomics analysis in the human cornea-on-a-chip for the determination of ocular drug toxicity
| Project/Area Number |
22K14548
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
ABDALKADER Rodi 立命館大学, 立命館グローバル・イノベーション研究機構, 助教 (20839964)
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Keywords | Cornea-on-a-chip / Microfluidic / Metabolomic / Drug toxicity |
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| Outline of Annual Research Achievements |
This research aims to identify metabolite markers related to drug toxicity using untargeted metabolomic analysis in a human corneal epithelium-on-a-chip. In the current fiscal year, an untargeted metabolic workflow that we previously developed was utilized to analyze extracellular metabolites in human induced pluripotent stem cells (hiPSCs) under early differentiation conditions. A total of 117 metabolites were successfully annotated in a small sample volume of one microliter. This optimized untargeted metabolomic method will be applied to the human corneal epithelium-on-a-chip in the next phase of the study.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
The progress of this research can be attributed to the successful optimization of the untargeted metabolomic method for detecting extracellular metabolites in both corneal epithelial cells and hiPSCs. Additionally, the establishment of functional corneal epithelial cells from hiPSCs will further facilitate the investigation of drug toxicity in the corneal epithelium-on-a-chip model with greater precision and accuracy.
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| Strategy for Future Research Activity |
To investigate changes in extracellular metabolites, the untargeted metabolomic method optimized in the previous stage will be applied in the human corneal epithelium-on-a-chip under drug treatment.
In parallel, changes in gene expression under drug treatment will also be investigated. This will be done using transcriptomic analysis to identify differentially expressed genes in response to drug treatment.
Finally, to validate the relationship between changes in extracellular metabolites and gene expression, correlation analysis will be performed to identify any significant associations between the two. These results will provide a more comprehensive understanding of drug toxicity related metabolite markers and their underlying mechanisms.
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| Causes of Carryover |
The budget allocated for this research project will be utilized to purchase reagents and human induced pluripotent stem cell culturing tools, as well as to cover expenses for attending international or domestic meetings and publishing the research findings. The applicant intends to utilize the budget within the current fiscal year to ensure the timely completion of the research project.
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