Development of Polyion Complex Vesicles with High Aspect Ratio

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K14735
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 35020:Polymer materials-related
• Research Institution: Kyoto University
• Principal Investigator: Fujita Seiya 京都大学, 工学研究科, 特定助教 (30824007)
• Project Period (FY): 2022-04-01 – 2024-03-31
• Keywords: ペプチド / ベシクル / タンパク質

Outline of Final Research Achievements

Tubular assemblies with semipermeability containing enzymes can be efficiently introduce enzymes into plants. In this research, we aimed to construct novel polyion complex vesicles (PICsomes) with a high aspect ratio, directed towards the introduction of enzymes into plants. Therefore, we explored the incorporation of multiple β-sheet-forming sequences into the PICsome structure. Various peptides were synthesized using solid-phase synthesis, and their secondary structures were evaluated to optimize the peptide sequences to be incorporated. As a result, it was suggested that repeating sequences of serine, which were found to form β-sheets, were optimal in terms of β-sheet strength and solubility.

Free Research Field

生体関連化学

Academic Significance and Societal Importance of the Research Achievements

酵素の直接導入は、遺伝子組み換え技術を使用しない植物の形質付与法として非常に高い関心がもたれている。一方で、酵素の導入効率が低いという問題があり、実用化に至っていない。今回、得られた知見から今後酵素を内包した高アスペクト比を有する新規ポリイオンコンプレックスベシクル(PICsome)の構築できれば、現在より高い効率で酵素を植物に導入できるようになると考えられる。したがって、本研究課題の結果により、遺伝子組み換え技術を使用しない植物の形質付与法の実用化に一歩近づいたと考え、今後の技術発展が期待される。