2023 Fiscal Year Annual Research Report
Creation and biological evaluation of nanoparticle-based artificial transcription factor
| Project/Area Number |
22K15249
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Keywords | NANO-TF / Silica nanoparticles / Mitochondrial factors / Cell Imaging / Molecular modeling / medaka egg imaging / Carbon quantum dots / Silicon quantum dots |
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| Outline of Annual Research Achievements |
We have developed SiQDs and CQDs for bio-imaging, as well as to prepare the artificial transcription factor, NANO-TF. The QDs we used were heavy metal-free materials without the use of cadmium and mercury. Instead, we used silicon with decene molecules for surface functionalization and Pluronic F127 for the water-soluble process. Additionally, we synthesized CQDs from waste biomass materials. We tested the cytotoxicity of both materials on various cell lines and Japanese medaka fish and found that a concentration of 1mg/mL did not cause any cytotoxic effects. We used these materials to develop NANO-TF. The NANO-TF units contain drugs and mitochondrial penetrating peptides, which enable them to penetrate inside the mitochondria.
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