Creation and biological evaluation of nanoparticle-based artificial transcription factor

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K15249
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 47010:Pharmaceutical chemistry and drug development sciences-related
• Research Institution: Kyoto University
• Principal Investigator: Chinnathambi Shanmugavel 京都大学, 高等研究院, 特定研究員 (60922341)
• Project Period (FY): 2022-04-01 – 2024-03-31
• Keywords: Quantum dots / Cell imaging / Transcription Factor / Medaka fish / reactive oxygen species / Molecular modeling

Outline of Final Research Achievements

We have developed water-soluble SiQDs and CQDs for bio-imaging, as well as to prepare the artificial transcription factor. Instead, we used silicon QDs with decene molecules for surface functionalization and Pluronic F127 for the water-soluble process. Additionally, we synthesized CQDs from biomass waste.We tested the cytotoxicity of both materials on various cell lines, including HeLa, A549, fibroblast, iPS, cardiomyocytes, and Japanese medaka fish. We used these materials to develop NANO-TF. The NANO-TF units contain drugs and mitochondrial penetrating peptides, which enable them to penetrate inside the mitochondria.

Free Research Field

Nanomedicine

Academic Significance and Societal Importance of the Research Achievements

当研究室において合成された量子ドットベースの NANO-TF は、ミトコンドリア膜を透過してがん細胞のミトコンドリア DNA に到達する能力を持っており、得られた結果は、QD ベースの薬物送達による癌細胞の破壊に大きく役立つ。