Mechanism elucidation of diabetic kidney glomerular lesion based on single-cell RNA sequence.

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K16223
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53040:Nephrology-related
• Research Institution: Kumamoto University
• Principal Investigator: Fujimoto Daisuke 熊本大学, 大学院生命科学研究部(医), 助教 (00896649)
• Project Period (FY): 2022-04-01 – 2024-03-31
• Keywords: 糖尿病性腎臓病 / 糸球体 / シングルセル解析 / 細胞連関

Outline of Final Research Achievements

This study aims to elucidate the mechanisms of diabetic kidney disease (DKD) based on glomerular single-cell RNA sequencing database. Our original single-cell protocol enabled us to explore several candidate genes which may be involved in DKD pathogenesis. We cretaed genetically modified animals and evaluated the renal phenotype, which could lead to the development of novel therapeutic strategy against DKD.

Free Research Field

腎臓内科学

Academic Significance and Societal Importance of the Research Achievements

糖尿病性腎臓病(DKD)は、末期腎不全に至る原因疾患として最多であり、根本的な治療法にも乏しいのが実情である。本研究はシングルセル解析により糸球体構成細胞種毎の解析が可能となり、病変形成に関与する遺伝子・パスウェイの探索及びそれらを標的としたDKD新規治療法の開発へ向けて大きな足がガリとなることが期待される。