2023 Fiscal Year Final Research Report
Mechanisms of M2 macrophages Induction by fibrin hydrogels and their application in apical periodontal tissue regeneration
| Project/Area Number |
22K17064
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 57030:Conservative dentistry-related
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Keywords | M2マクロファージ / フィブリンゲル / 骨再生 / 炎症制御 |
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| Outline of Final Research Achievements |
Our research revealed that by implanting fibrin hydrogels into bone defect sites, M2 macrophages were induced at the wound site, promoting bone regeneration by acting towards inflammation suppression. Additionally, fibrin gel has the function of transitioning the tissue state during the acute phase of inflammation, specifically in environments where LPS is present in the tissue, to an inflammation-suppressing state dominated by M2 macrophages. Furthermore, RNA-seq was performed on odontoblast cell lines co-cultured with fibrin hydrogels. The results showed that the genes expressed in the cell lines co-cultured with fibrin gel were significantly different from those in the standard culture method, suggesting a potential impact on inflammation and differentiation.
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| Free Research Field |
歯内治療学
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| Academic Significance and Societal Importance of the Research Achievements |
患者の血液からフィブリンゲルを作成して再生治療に用いた症例が報告されているが、治療効果が不安定なため現状では再生医用材料としては不十分である。フィブリンゲルによる安定した効果が得られない理由として、組織創傷部位へのフィブリンゲルの作用機序が十分に解析されていないことが挙げられる。しかし我々の研究結果により、vitroで作成したフィブリンゲルでも強いM2マクロファージ誘導能があり、それによって骨再生が増強されるということが示唆された。この結果はフィブリンゲルの再生医用材料への応用においても有意義であるが、フィブリンの生体機能の解明という点でも学術的意義がある。
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