Elucidation of bone regeneration mechanism of SHED-CM and exosomes on bone regeneration and clinical application for cleft palate bone regeneration treatment

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K17229
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Hiroshima University
• Principal Investigator: HIRAKI TOMOKA 広島大学, 医系科学研究科(歯), 研究員 (70881275)
• Project Period (FY): 2022-04-01 – 2024-03-31
• Keywords: SHED / 間葉系幹細胞 / 骨再生 / 培養上清

Outline of Final Research Achievements

In this study, we focused on the bone regeneration potential of deciduous dental pulp-derived mesenchymal stem cells (SHED) and SHED culture supernatant (SHED-CM). The effect of SHED-CM transplantation on bone regeneration was examined. In addition, to elucidating the humoral factors that are valuable for bone regeneration during SHED-CM, exosomal components of SHED-CM contained within SHED-CM were explored. It was revealed that SHED and SHED-CM transplantation had the bone regeneration.
We elucidated the humoral factors contained in SHED-CM. Furthermore, we elucidated that adding SHED-CM to osteoblasts and bone marrow-derived mesenchymal stem cells enhanced cell proliferative capacity and the expression of osteogenesis-related factors. These findings indicate the potential benefits of SHED-CM in bone tissue regeneration.

Free Research Field

骨再生

Academic Significance and Societal Importance of the Research Achievements

近年、間葉系幹細胞が分泌するサイトカインのパラクライン作用が注目され、幹細胞培養上清を用いた組織再生が報告されているものの、その作用機序については不明な点が多い。本研究では、SHED-CMに含有される液性成分について解析し、移植を行うことで良好な骨再生を生じることを明らかとした。また、骨芽細胞等に対して、SHED-CM添加は、細胞増殖能や骨形成能を亢進させることを解明した。今回得られた研究結果によって、低侵襲で良好な骨再生療法を確立することで、患者の負担を軽減できることは、社会的観点から重要な社会的意義があると考えられる。