Exploration of highly functional exosomes using a continuous media recovery system and its application to drug delivery

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K19384
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 47:Pharmaceutical sciences and related fields
• Research Institution: University of Shizuoka
• Principal Investigator: Koide Hiroyuki 静岡県立大学, 薬学部, 准教授 (60729177)
• Co-Investigator(Kenkyū-buntansha): 奥 直人 帝京大学, 薬学部, 教授 (10167322) ; 疋田 智也 愛知県がんセンター(研究所), 腫瘍制御学分野, 主任研究員 (20600935)
• Project Period (FY): 2022-06-30 – 2024-03-31
• Keywords: エクソソーム / 薬物送達 / がん

Outline of Final Research Achievements

In this study, we developed a device that continuously pumps and collects medium during cell culture, and attempted to collect highly functional exosomes produced by cells because some exosomes are taken up by the cells after production, immediately. As a result, the number of exosomes was significantly increased by continuous collection of medium compared to the conventional method. Furthermore, when the exosomes were added to cells and the amount of cellular uptake was measured, the amount of cellular uptake of exosomes was markedly increased compared to the conventional exosomes. These results indicate that exosomes collected by the developed device contain highly functional exosomes. From hese results, continuous recovery of the culture medium is one of the method for the collection of highly functional exosomes.

Free Research Field

薬物送達

Academic Significance and Societal Importance of the Research Achievements

エクソソームが細胞間のコミュニケーションツールとして考えられていこう、今日までエクソソーム研究が盛んに行われてきた。しかし、これまでエクソソームは数日間の静置培養液をEV回収源としてきた。そのため、高い細胞取り込み能を示す機能的エクソソームを回収することは困難であり、これまでは主に細胞への取り込み能の低い非機能的エクソソームを回収し、解析してきた可能性が高い。そのため、本研究により回収した高機能のエクソソームの生物学的機能解析や膜タンパク質、核酸などの内封物質を解析することで、難治性疾患のメカニズムの解明を通した新薬開発やバイオマーカー開発につながることから、学術的、社会的意義は高い。