Anti-tumor effects of arginine in three-dimensional cultured EBV-positive lymphoma cells

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K20769
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0803:Pathology, infection/immunology, and related fields
• Research Institution: Shiga University of Medical Science
• Principal Investigator: Noujima Mai 滋賀医科大学, 医学部, 助教 (20961783)
• Project Period (FY): 2022-08-31 – 2024-03-31
• Keywords: アルギニン / EBウイルス / 三次元培養 / リンパ腫

Outline of Final Research Achievements

Two-dimensional culture of FL18 (EBV-negative lymphoma cell line) in arginine- and non-arginine-supplemented medium showed a trend toward a decrease in cell proliferative capacity in arginine concentration-dependent manner. This result suggests that arginine has a "tumor suppressive effect" in EBV-negative lymphomas.
We succeeded in three-dimensional culture of FL18 and obtained optimal culture conditions. We divided the FL18 into four groups: (1) two-dimensional culture in arginine-free medium, (2) two-dimensional culture in arginine-added medium, (3) three-dimensional culture in arginine-free medium, and (4) three-dimensional culture in arginine-added medium. The metabolomic analysis showed that the addition of arginine altered specific metabolic systems.

Free Research Field

病理学

Academic Significance and Societal Importance of the Research Achievements

リンパ腫細胞株におけるアルギニン投与効果の検証は，リンパ腫の腫瘍微小環境の理解の一助となる．また，リンパ腫だけでなくその他悪性腫瘍においても，代謝改変に基づく新規治療法の発見に貢献しうると思われる．