Innovation of genotoxicity tests - DNA damage, Mutation, Chromosome -

2015 Fiscal Year Final Research Report

• Project/Area Number: 23221006
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kyoto University
• Principal Investigator: Matsuda Tomonari 京都大学, 工学(系)研究科(研究院), 准教授 (50273488)
• Co-Investigator(Kenkyū-buntansha): Ikura Tsuyoshi 京都大学, 放射線生物学研究センター, 准教授 (70335686)
• Co-Investigator(Renkei-kenkyūsha): Adachi Jun 国立研究開発法人医薬基盤・健康・栄養研究所, 創薬基盤研究部, プロジェクト研究員 (20437255)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: 突然変異 / DNA損傷 / 染色体異常 / 遺伝毒性試験 / 1分子リアルタイムDNAシーケンサー

Outline of Final Research Achievements

Genotoxicity tests are essential for pre-marketing hazard assessment of newly-developed chemicals. Recent development of analytical equipment and cell biology allow us to make innovation in this area. In this study, we tried to develop innovative genotoxicity tests which focused on DNA damage, mutation and mechanism of chromosomal aberration. As for DNA damage, we modified the protocol of DNA adductome method to make it faster and more sensitive. We also developed a new mutation assay using a single molecule real time DNA sequencer, enabling direct monitoring of mutation frequency in DNA of any samples. We also developed methods to evaluate inhibition of DNA damage response which is the important mechanism for DNA damage independent chromosomal aberration.

Free Research Field

環境変異原