維管束幹細胞の発生運命制御機構の解明

2014 Fiscal Year Annual Research Report

• Project/Area Number: 23227001
• Research Institution: The University of Tokyo
• Principal Investigator: 福田 裕穂 東京大学, 理学(系)研究科(研究院), 教授 (10165293)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: 成長生理 / 植物 / 発生・分化

Outline of Annual Research Achievements

本研究の目的は、植物成長の鍵となる植物メリステムの組織構築機構を、幹細胞の発生運命制御の解明を通して明らかにすることにある。今年度は、TDIF機能を中心に維管束幹細胞の維持機構と、維管束幹細胞の成立機構、さらには維管束幹細胞からの木部細胞分化機構を、以下に示す３つの観点から解析した。

１．WOX4の下流因子の解明：　WOX4ターゲット候補遺伝子について、その機能をT-DNA挿入変異体を用いて解析したが、顕著な表現型は得られなかった。そこで、更なるターゲット遺伝子の探索のために、チップシークエンスを行うこととし、その準備を行った。
２．ブラシノステロイドシグナル伝達とTDIFシグナル伝達のクロストークの解析：　ブラシノステロイドシグナルとTDIFがGSK3-BES1経路を介してクロストークすることが明らかになったので、これらの２つのシグナル系がどのようにGSK3-BES1経路の制御を行っているのかを、BES1ファミリーを中心に解析した。その結果、２重変異体の作出に成功した。
３．leaf disk培養を用いた維管束幹細胞成立の解析：　私たちが昨年までに開発したleaf disk培養系では、葉肉細胞から維管束幹細胞の分化を経て、木部細胞・篩部細胞が分化する。そこで、この実験系を用いて、マイクロアレー解析を行い、葉肉細胞から維管束幹細胞への分化に関わる新規因子の同定を行った。その結果、多くの新規の遺伝子が同定され、そのうちには、NACタイプの転写因子も含まれていた。また、維管束細胞成立の研究において、新規のシグナル伝達分子の同定に成功した。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

３つの課題とも順調に成果が出ており、とりわけ、「３」に関しては、新規の因子の同定に成功した。

Strategy for Future Research Activity

今後、維管束幹細胞の維持機構、維管束幹細胞の成立機構、維管束幹細胞からの木部細胞分化機構の解明を、特にleaf disk培養を中心にして進め、そのシグナル伝達のアウトラインを明らかにする。

Research Products

• [Journal Article] A novel system for xylem cell differentiation in Arabidopsis thaliana. (2015)
  • Author(s): Kondo, Y., Fujita, T., Sugiyama, M., and Fukuda, H.
  • Journal Title: Mol. Plant Volume: 8 Pages: 612-621
  • DOI: 10.1093/mp/ssu122
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Novel coiled-coil proteins regulate exocyst association with cortical microtubules in xylem cells via conserved oligomeric Golgi-complex 2 protein. (2015)
  • Author(s): Oda, Y. Iida, Y., Ebine, K., Ueda, T., and Fukuda, H.
  • Journal Title: Plant Cell Physiol. Volume: 56 Pages: 277-286
  • DOI: 10.1093/pcp/pcu197
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] VAN4 encodes a putative TRS120 that is required for normal cell growth and vein development in Arabidopsis. (2014)
  • Author(s): Naramoto, S., Nodzynski, T., Dainobu, T., Takatsuka, H., Okada, T., Friml, J., and Fukuda, H.
  • Journal Title: Plant Cell Physiol. Volume: 55 Pages: 750-763
  • DOI: 10.1093/pcp/pcu012
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Plant GSK3s regulate stem cell differentiation downstream of TDIF-TDR signalling. (2014)
  • Author(s): Kondo, Y., Ito, T., Nakagami, H, Hirakawa, Y., Saito, M., Tamaki, T., Shirasu, K., and Fukuda, H.
  • Journal Title: Nature Com. Volume: 5 Pages: 3504
  • DOI: 10.1038/ncomms4504
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis thaliana. (2014)
  • Author(s): Naramoto, S., Otegui, M. S., Kutsuna N., de Rycke, R., Dainobu, T., Karampelias, M., Fujimoto, M., Feraru, E., Miki, D., Fukuda, H., Nakano, A. and Friml, J.
  • Journal Title: Plant Cell Volume: 26 Pages: 3062-3076
  • DOI: 10.1105/tpc.114.125880
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] CLE6 expression recovers gibberellin deficiency to promote shoot growth in Arabidopsis. (2014)
  • Author(s): Bidadi, H., Matsuoka, K., Sage-Ono, K., Fukushima, J., Pitaksaringkarn, W., Asahina, M., Yamaguchi, S., Sawa, S., Fukuda, H., Matsubayashi, Y., Ono, M., and Satoh, S.
  • Journal Title: Plant J. Volume: 78 Pages: 241-252
  • DOI: 10.1111/tpj.12475
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A bHLH complex activates vascular cell division via cytokinin action in root apical meristem. (2014)
  • Author(s): Ohashi-Ito, K., Saegusa, M., Iwamoto, K., Oda, Y., Katayama, H., Kojima. M., Sakakibara, H., and Fukuda, H.
  • Journal Title: Curr. Biol. Volume: 24 Pages: 2053-2058
  • DOI: 10.1016/j.cub.2014.07.050
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Heterotrimeric G proteins control stem cell proliferation through CLAVATA signaling in Arabidopsis. (2014)
  • Author(s): Ishida, T. Tabata, R., Yamada, M., Aida, M., Mitsumasu, K., Fujiwara, M., Yamaguchi, K., Shigenobu, S., Higuchi, M., Tsuji, H., Shimamoto, K., Hasebe, M., Fukuda, H., and Sawa, S.
  • Journal Title: EMBO reports Volume: 15 Pages: 1202-1209
  • DOI: 10.15252/embr.201438660
  • Peer Reviewed
• [Journal Article] 134Cs and 137Cs levels in a grassland, 32 km northwest of the Fukushima 1 Nuclear Power Plant, measured for two seasons after the fallout. (2014)
  • Author(s): Terashima, I., Shiyomi, M., and Fukuda, H.
  • Journal Title: Journal of Plant Research Volume: 127 Pages: 43-50
  • DOI: 10.1007/s10265-013-0608-9
  • Peer Reviewed
• [Journal Article] As plant scientists who faced the Fukushima nuclear disaster. (2014)
  • Author(s): Mimura, T., Fujiwara, T., and Fukuda, H.
  • Journal Title: Journal of Plant Research Volume: 127 Pages: 3-4
  • DOI: 10.1007/s10265-013-0606-y
  • Peer Reviewed
• [Journal Article] Initiation of vascular development. (2014)
  • Author(s): Ohashi-Ito, K. and Fukuda, H.
  • Journal Title: Physiol. Plant. Volume: 151 Pages: 142-146
  • DOI: 10.1111/ppl.12111
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Regulation of xylem cell fate. (2014)
  • Author(s): Kondo, Y., Tamaki, T. and Fukuda, H.
  • Journal Title: Frontiers in Plant Sci. Volume: 5 Pages: 315
  • DOI: 10.3389/fpls.2014.00315
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Emerging roles of small GTPases in secondary cell wall development. (2014)
  • Author(s): Oda Y, Fukuda H.
  • Journal Title: Frontiers in Plant Sci. Volume: 5 Pages: 428
  • DOI: 10.3389/fpls.2014.00428
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Endogenous peptide ligand-receptor systems for diverse signaling networks in plants. (2014)
  • Author(s): Endo S, Betsuyaku S, Fukuda H.
  • Journal Title: Curr. Opin. Plant Biol. Volume: 21 Pages: 140-146
  • DOI: 10.1016/j.pbi.2014.07.011
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Xylem. (2014)
  • Author(s): Ohashi-Ito, K. and Fukuda, H.
  • Journal Title: Curr. Biol. Volume: 24 Pages: R1149
  • Peer Reviewed
• [Presentation] Regulation of vascular stem cell fates by a peptide and a steroid hormone. (2014)
  • Author(s): Fukuda, H.
  • Organizer: The 38th Naito Conference on Molecular-based biological systems.
  • Place of Presentation: シャトレーゼ ガトーキングダム サッポロ（北海道・札幌）
  • Year and Date: 2014-10-07 – 2014-10-10
  • Int'l Joint Research / Invited
• [Presentation] Regulatory mechamisms controlling vascular stem cell differentiation -- Elucidation of TDIF-TDR signaling cascade --. (2014)
  • Author(s): Kondo, Y. and Fukuda, H.
  • Organizer: The 38th Naito Conference on Molecular-based biological systems.
  • Place of Presentation: シャトレーゼ ガトーキングダム サッポロ（北海道・札幌）
  • Year and Date: 2014-10-07 – 2014-10-10
  • Int'l Joint Research
• [Presentation] Polarized localization of PIN-FORMED2 (PIN2) auxin efflux carrier protein and its microdomain formation in plant cells. (2014)
  • Author(s): Naramoto, S., Furutani, M., Langowski, L., Li, H., Fukuda, H., Friml, J., Tasaka, M., and Nakano, A.
  • Organizer: The 38th Naito Conference on Molecular-based biological systems.
  • Place of Presentation: シャトレーゼ ガトーキングダム サッポロ（北海道・札幌）
  • Year and Date: 2014-10-07 – 2014-10-10
  • Int'l Joint Research
• [Presentation] The molecular basis of secondary cell wall patterning: self-organization by the dynamic interplay between Rho GTPase and cortical cytoskeleton. (2014)
  • Author(s): Oda, Y., Nagashima, Y., Sugiyama, Y., and Fukuda, H.
  • Organizer: The 38th Naito Conference on Molecular-based biological systems.
  • Place of Presentation: シャトレーゼ ガトーキングダム サッポロ（北海道・札幌）
  • Year and Date: 2014-10-07 – 2014-10-10
  • Int'l Joint Research
• [Presentation] Divergent functions of CLE peptides: from cell fate determination to the regulation of high temperature resistance. (2014)
  • Author(s): Fukuda, H.
  • Organizer: 2nd European Workshop on Peptide Signalling in Plants
  • Place of Presentation: Regensburg, Germany
  • Year and Date: 2014-09-03 – 2014-09-05
  • Int'l Joint Research / Invited
• [Presentation] Intracellular signalling governing vascular stem cell fates. (2014)
  • Author(s): Fukuda, H. and Kondo, Y.
  • Organizer: Plenary symposium “Plant Signaling” American Society of Plant Biology Annual Meeting
  • Place of Presentation: Oregon, USA
  • Year and Date: 2014-07-12 – 2014-07-17
  • Int'l Joint Research / Invited
• [Presentation] Plant GSK3s regulate xylem cell differentiation downstream of TDIF-TDR signaling. (2014)
  • Author(s): Fukuda, H.
  • Organizer: Mini symposium “Recent Advances in Plant and Microbial Biology” IPMB
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2014-06-10
  • Int'l Joint Research / Invited
• [Book] Plant cell wall patterning and cell shape (2014)
  • Author(s): Fukuda, H (ed.)
  • Total Pages: 415
  • Publisher: Wiley-Blackwell publishing
• [Remarks] 東京大学大学院理学系研究科生物科学専攻生体制御研究室
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/seigyo/lab.html