2013 Fiscal Year Final Research Report
The role of Rho-mDia siganling in tissue architecture and homeostasis in the body
| Project/Area Number |
23229003
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
NARUMIYA Shuh 京都大学, 医学(系)研究科(研究院), 教授(特任) (70144350)
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| Project Period (FY) |
2011-05-31 – 2014-03-31
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| Keywords | mDia / actin / Liprin / neuroepithelial polarity / neuronal migration / axon guidance / cytokinesis / tissue architecture |
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| Research Abstract |
Here we examined action and regulation of mDia, a Rho-regulated actin nucleator/polymerization factor, at the molecular and cellular level and the role of each mDia isoform in tissue architecture and maintenance in the body. In the former, we discovered that mDia catalyzes actin polymerization by rotating along the pitch of actin filament and that Liprin-alpha negatively regulating mDia activity by trapping mDia in the cytosol. In the latter, we used mDia-deficient KO mice and discovered that mDia contribute to shaping and maintenance of architecture of the brain by regulating the neuroepithelial polarity, tangential migration of inhibitory neuron precursors and axon guidance of the corticospinal tract neurons. We also discovered that mDia2 regulates cytokinesis of erythroblasts and contributes to maintenace of the hematopoietic system. In this work, we further elucidated how mDia-induced actin cytoskeleton functions in each of these in vivo processes.
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[Journal Article] Loss of a Rho-Regulated Actin Nucleator, mDia2, Impairs Cytokinesis during Mouse Fetal Erythropoiesis2013
Author(s)
Watanabe S, De Zan T, Ishizaki T, Yasuda S, Kamijo H, Yamada D, Aoki T, Kiyonari H, Kaneko H, Shimizu R, Yamamoto M, Goshima G, Narumiya S
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Journal Title
Cell Rep
Volume: 5(4)
Pages: 926-932
DOI
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[Journal Article] A role for mDia, a Rho-regulated actin nucleator, in tangential migration of interneuron precursors2012
Author(s)
Shinohara R, Thumkeo D, Kamijo H, Kaneko N, Sawamoto K, Watanabe K, Takebayashi H, Kiyonari H, Ishizaki T, Furuyashiki T, Narumiya S
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Journal Title
Nature Neurosci
Volume: 15(3)
Pages: 373 -380
DOI
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[Journal Article] Deficiency of mDia, an actin nucleator, disrupts integrity of neuroepithelium and causes periventricular dysplasia2011
Author(s)
Thumkeo D, Shinohara R, Watanabe K, Takebayashi H, Toyoda Y, Tohyama K, Ishizaki T, Furuyashiki T, Narumiya S
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Journal Title
PLoS One
Volume: 6(9)
Pages: e25465
DOI
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