2013 Fiscal Year Final Research Report
The elucidation of pathogenesis of developmental disorders induced by maternal nutritional condition in the fetal stage.
| Project/Area Number |
23240062
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
WAKANA Shigeharu 独立行政法人理化学研究所, バイオリソースセンター, チームリーダー (90192434)
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| Co-Investigator(Kenkyū-buntansha) |
KUBOTA Takeo 山梨大学, 医学工学総合研究部, 教授 (70293511)
FURUICHI Teiichi 東京理科大学, 理工学部応用生物学科, 教授 (50219094)
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| Project Period (FY) |
2011-05-31 – 2014-03-31
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| Keywords | DOHaD / 発達障害 / 行動解析 / 実験動物学 / 胎児期栄養 / CAPSタンパク質 / 葉酸 / 遺伝子発現 |
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| Research Abstract |
The Developmental Origins of Health and Disease:DOHaD paradigm is a concept that in utero experiences reprogram an individual for later-in-life risk of diseases. In this study, we conducted experiments to validate the DOHaD theory in the developmental disorder with well-controlled experimental procedures using mouse model. We provided AIN93G as control diet and low-protein diet (LP) to pre-pregnant and pregnant females. After birth, behavioral phenotypes of offspring, gene expression level in brain, and genomic methylation levels in promoters of genes were examined. The offspring which were exposed to malnutrition in utero exhibited increased activity in the home cage, decreased contact to novel object, and decreased social investigation. The adult offspring of LP group group exhibited different pattern of mRNA expression and genomic methylation pattern relative to AIN93G group. However, no direct interaction between expression levels and methylation levels of genes were detected.
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Research Products
(27 results)
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[Journal Article] CAPS1 deficiency perturbs dense-core vesicle trafficking and Golgi structure and reduces presynaptic release probability in the mouse brain2013
Author(s)
Sadakata T, Kakegawa W, Shinoda Y, Hosono M, Katoh-Semba R, Sekine Y, Sato Y, Tanaka M, Iwasato T, Itohara S, Furuyama K, Kawaguchi Y, Ishizaki Y, Yuzaki M, Furuichi T
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Journal Title
J. Neurosci
Volume: 33
Pages: 17326-17334
DOI
Peer Reviewed
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[Journal Article] Comparison of genomic and epigenomic expression in monozygotic twins discordant for Rett syndrome2013
Author(s)
Miyake K, Yang C, Minakuchi Y, Ohori K, Soutome M, Hirasawa T, Kazuki Y, Adachi N, Suzuki S, Itoh M, Goto Y, Andoh T, Kurosawa H, Akamatsu W, Oyama M, Okano H, Oshimura M, Sasaki M, Toyoda A, Kubota T
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Journal Title
PLoS ONE
Volume: 8
Pages: e66729
DOI
Peer Reviewed
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[Presentation] マウスモデルを用いたDOHaD 仮説の実証2 : 母体低栄養が新生仔の行動表現型に与える影響2013
Author(s)
古瀬民生, 幸田尚, 串田知子, 柏村実生, 山田郁子, 金田秀貴, 小林喜美男, 石野史敏, 古市貞一, 久保田健夫, 若菜茂晴
Organizer
第60回日本実験動物学会総会
Place of Presentation
つくば国際会議場(つくば市)
Year and Date
2013-05-15
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