A role for noncoding RNA in recognition of homologous chromosomes

2013 Fiscal Year Final Research Report

• Project/Area Number: 23247003
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Osaka University
• Principal Investigator: HIRAOKA Yasushi 大阪大学, 生命機能研究科, 教授 (10359078)
• Co-Investigator(Renkei-kenkyūsha): ASAKAWA Haruhiko 大阪大学, 大学院生命機能研究科, 助教 (70399533) ; CHIKASHIGE Yuji (独)情報通信研究機構, 未来ICT 研究所, 主任研究員 (60359081) ; DING Da-qiao (独)情報通信研究機構, 未来ICT 研究所, 主任研究員 (50359080) ; OBUSE Chikashi 北海道大学, 先端生命科学研究院, 教授 (00273855)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 染色体 / 非コードRNA

Research Abstract

Meiosis is an essential process for sexually-reproducing organisms. During meiosis, homologous chromosomes pair with each other and undergo homologous recombination, which is crucial for proper segregation of chromosomes. In the fission yeast Schizosaccharomyces pombe, clustering of telomeres promotes homologous chromosome pairing. However, the mechanisms by which chromosomes recognize their homologous partners remain unclear. We demonstrate that the sme2 gene encodes a meiosis-specific noncoding RNA that mediates homologous recognition in S. pombe. The sme2 RNA transcripts accumulate at their respective gene loci, and greatly enhances pairing of homologous loci: deletion of the sme2 sequence eliminates this robust pairing while transposition to other chromosomal sites confers the robust pairing at those sites. Results indicate that RNA transcripts retained on the chromosome play a role in recognition of homologous chromosomes in S. pombe.

Research Products

• [Journal Article] Characterization of nuclear pore complex components in fission yeast Schizosaccharomyces pombe (2014)
  • Author(s): Asakawa H, Yang H-J, Yamamoto TG, Ohtsuki C, Chikashige Y, Sakata-Sogawa K, Tokunaga M, Iwamoto M, Hiraoka Y, Haraguchi T
  • Journal Title: Nucleus. Volume: 5(in press)
  • DOI: 10.4161/nucl.28487
  • Peer Reviewed
• [Journal Article] The role of chromosomal retention of noncoding RNA in meiosis (2013)
  • Author(s): Ding D-Q, Haraguchi T, Hiraoka Y.
  • Journal Title: Chromosome Res Volume: 21 Pages: 665-672
  • DOI: 10.1007/s10577-013-9389-1
  • Peer Reviewed
• [Journal Article] Mediator Directs Co-transcriptional Heterochromatin Assembly by RNA Interference-Dependent and - Independent Pathways (2013)
  • Author(s): Oya E, Kato H, Chikashige Y, Tsutsumi C, Hiraoka Y, Murakami Y
  • Journal Title: PLoS Genet Volume: 9 Pages: e1003677
  • DOI: 10.1371/journal.pgen.1003677
  • Peer Reviewed
• [Journal Article] Microtubule -organizing center formation at telomeres induces meiotic telomere clustering (2013)
  • Author(s): Yoshida M, Katsuyama S, Tateho K, Nakamura H, Miyoshi J, Ohba T, Matsuhara H, Miki F, Okazaki K, Haraguchi T, Niwa O, Hiraoka Y, Yamamoto A
  • Journal Title: J. Cell Biol Volume: 200 Pages: 385-395.
  • DOI: 10.1083/jcb.201207168
  • Peer Reviewed
• [Journal Article] Purification and characterization of the fission yeast telomere clustering factors, Bqt1 and Bqt2 (2013)
  • Author(s): Ichikawa Y, Kagawa W, Saito K, Chikashige Y, Haraguchi T, Hiraoka Y, Kurumizaka H
  • Journal Title: Protein Expr Purif. Volume: 88 Pages: 207-213
  • DOI: 10.1016/j.pep.2013.01.006.
  • Peer Reviewed
• [Journal Article] Chromosomally-retained RNA mediates homologous pairing (2012)
  • Author(s): Ding D-Q, Haraguchi T, Hiraoka Y
  • Journal Title: Nucleus Volume: 3 Pages: 516-519
  • DOI: 10.4161/nucl.22732
  • Peer Reviewed
• [Journal Article] Telomere-Nuclear Envelope Dissociation Promoted by Rap1 Phosphorylation Ensures Faithful Chromosome Segregation (2012)
  • Author(s): Fujita I, Nishihara Y, Tanaka M, Tsujii H, Chikashige Y, Watanabe Y, Saito M, Ishikawa F, Hiraoka Y, Kanoh J
  • Journal Title: Curr. Biol Volume: 22 Pages: 1932-1937
  • DOI: 10.1016/j.cub.2012.08.019
  • Peer Reviewed
• [Journal Article] The CCR4-NOT Complex Is Implicated in the Viability of Aneuploid Yeasts (2012)
  • Author(s): Tange Y, Kurabayashi A, Goto B, Hoe K-L, Kim D-U, Park H-O, Hayles J, Chikashige Y, Tsutumi C, Hiraoka Y, Yamao F, Nurse P, Niwa O
  • Journal Title: PLoS Genetics Volume: 8 Pages: e1002776
  • DOI: 10.1371/journal.pgen.1002776
  • Peer Reviewed
• [Journal Article] Meiosis-specific non-coding RNA mediates robust pairing of homologous chromosomes in meiosis (2012)
  • Author(s): Ding D-Q , Okamasa K, Yamane M, Tsutsumi C, Haraguchi T, Yamamoto M, and Hiraoka Y
  • Journal Title: Science Volume: 336 Pages: 732-736
  • DOI: 10.1126/science.1219518
  • Peer Reviewed
• [Journal Article] Hexanucleotide motifs mediate recruitment of the RNA elimination machinery to silent meiotic genes (2012)
  • Author(s): Yamashita A, Shichino Y, Tanaka H, Hiriart E, Touat-Todeschini L, Vavasseur A, Ding D-Q, Hiraoka Y, Verdel A, Yamamoto M
  • Journal Title: Open Biology Volume: 2 Pages: 120014
  • DOI: 10.1098/rsob.120014
  • Peer Reviewed
• [Journal Article] A mutation of the fission yeast EB1 overcomes negative regulation by phosphorylation and stabilizes microtubules (2012)
  • Author(s): Iimori M, Ozaki K, Chikashige Y, Habu T, Hiraoka Y, Maki T, Hayashi I, Obuse C, Matsumoto T
  • Journal Title: Exp Cell Res. Volume: 318 Pages: 262-275
  • DOI: 10.1016/j.yexcr.2011.11.006
  • Peer Reviewed
• [Journal Article] Physical breakdown of the nuclear envelope is not necessary for breaking its barrier function (2011)
  • Author(s): Asakawa H, Hiraoka Y, Haraguchi T
  • Journal Title: Nucleus Volume: 2 Pages: 523-526
  • DOI: 10.4161/nucl.2.6.16117
  • Peer Reviewed
• [Journal Article] Inner nuclear membrane protein Ima1 is dispensable for intranuclear positioning of centromeres (2011)
  • Author(s): Hiraoka Y, Maekawa H, Asakawa H, Chikashige Y, Kojidani T, Osakada H, Matsuda A, Haraguchi T
  • Journal Title: Genes Cells Volume: 16 Pages: 1000-1011
  • DOI: 10.1111/j.1365-2443.2011.01544.x
  • Peer Reviewed
• [Journal Article] Aneuploidy drives genomic instability in yeast (2011)
  • Author(s): Sheltzer JM, Blank HM, Pfau SJ, Tange Y, George BM, Humpton TJ, BritoIL, Hiraoka Y, Niwa O, Amon A
  • Journal Title: Science Volume: 333 Pages: 1026-30
  • DOI: 10.1126/science.1206412
  • Peer Reviewed
• [Journal Article] Nuclear translocation of RanGAP1 coincides with virtual nuclear envelope breakdown in fission yeast meiosis (2011)
  • Author(s): Asakawa H, Hiraoka Y, Haraguchi T
  • Journal Title: Communicative and Integrative Biology Volume: 4 Pages: 312-314
  • DOI: 10.4161/cib.4.3.14808
  • Peer Reviewed
• [Presentation] Behaviors and structures of neocentromeres during meiosis in fission yeast (2013)
  • Author(s): Ogiyama Y, Asakawa H, Yamamoto TG, Haraguchi T, Ishii K, and Hiraoka Y
  • Organizer: EMBO Conference on Meiosis
  • Place of Presentation: Hotel Radisson Blu, Radebeul-Dresden, Germany
  • Year and Date: 2013-09-18
• [Presentation] Fission yeast nuclear membrane proteins that affect chromosome movements (2013)
  • Author(s): Tange Y, Asakawa H, Yang H-J, Chikashige Y, Haraguchi T, Hiraoka Y
  • Organizer: EMBO Conference on Fission Yeast
  • Place of Presentation: Senate House, London, UK
  • Year and Date: 2013-06-29
• [Presentation] Inner nuclear membrane proteins that affect chromosome dynamics in fission yeast (2013)
  • Author(s): Hiraoka Y
  • Organizer: Gordon Research Conference "Chromosome Dynamics"
  • Place of Presentation: Renaissance Tuscany Il Ciocco Resort, Lucca (Barga),Italy
  • Year and Date: 2013-05-27
  • Invited
• [Presentation] Dynamic Organization of Chromosomes In Fission Yeast (2013)
  • Author(s): Hiraoka Y
  • Organizer: Joint Conference of HGM 2013 and 21st International Congress of Genetics
  • Place of Presentation: The Sands Expo and Convention Center, Marina Bay Sands, Singapore
  • Year and Date: 2013-04-17
  • Invited
• [Presentation] Live-cell imaging of chromosome dynamics in fission yeast (2013)
  • Author(s): Hiraoka Y
  • Organizer: 1st International Symposium of the. Mathematics on Chromatin Live Dynamics
  • Place of Presentation: Hiroshima University, Japan
  • Year and Date: 2013-03-16
  • Invited
• [Presentation] 核内RNAボディーが相同染色体の認識に果たす役割 (2012)
  • Author(s): 平岡泰、丁大橋、原口徳子
  • Organizer: 第30回染色体ワークショップ・第11回核ダイナミクス研究会
  • Place of Presentation: 兵庫県立淡路夢舞台国際会議場、兵庫県
  • Year and Date: 2012-12-19
• [Presentation] 減数分裂特異的な非コードRNAが相同染色体を認識する (2012)
  • Author(s): 平岡泰、丁大橋、原口徳子
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡、福岡県
  • Year and Date: 2012-12-12
  • Invited
• [Presentation] RNA bodies on the chromosome play a role in pairing homologous chromosomes (2012)
  • Author(s): Hiraoka Y, Ding D-Q, Chikashige Y, Haraguchi T
  • Organizer: Cold Spring Harbor Laboratory Meeting, Dynamic Organization of Functional Nucleus
  • Place of Presentation: NEW YORK, USA
  • Year and Date: 2012-09-30
• [Presentation] 減数分裂特異的な非コードRNAが相同染色体の対合を促進する (2012)
  • Author(s): 平岡泰、丁大橋、原口徳子
  • Organizer: 第50回日本生物物理学会
  • Place of Presentation: 名古屋大学東山キャンパス、愛知県
  • Year and Date: 2012-09-23
  • Invited
• [Presentation] The Nuclear Envelope in Meiosis (2012)
  • Author(s): Hiraoka Y
  • Organizer: The 20th Jerusalem School in Life Sciences"Nuclear Organization, Dynamics and Activity"
  • Place of Presentation: Israel Institute for Advanced Studiesat the Hebrew University, Israel
  • Year and Date: 2012-07-23
  • Invited
• [Presentation] Meiosis-specific non-coding RNA in homologous chromosome pairing (2012)
  • Author(s): Hiraoka Y
  • Organizer: Gordon Research Conference "Meiosis"
  • Place of Presentation: Colby-Sawyer College,USA
  • Year and Date: 2012-06-06
  • Invited
• [Presentation] Meiosis-specific non-coding RNA mediates pairing of homologous chromosomes in meiosis (2012)
  • Author(s): Hiraoka Y
  • Organizer: Gordon Research Conference "Chromatin Structure & Function"
  • Place of Presentation: Colby-Sawyer College, USA
  • Year and Date: 2012-05-09
• [Presentation] Meiosis-specific non-coding RNA mediates robust pairing of homologous chromosomes in meiosis (2011)
  • Author(s): Hiraoka Y, Ding D-Q, Haraguchi T
  • Organizer: The EMBO Conference on Meiosis (2nd)
  • Place of Presentation: Capaccio/ Paestum, Italy
  • Year and Date: 2011-09-19
  • Invited
• [Presentation] Meiosis-specific non-coding RNA mediates robust pairing of homologous chromosomes in meiosis (2011)
  • Author(s): Hiraoka Y
  • Organizer: Gordon Research Conference "Chromosome Dynamics"
  • Place of Presentation: Mount Snow Resort, USA
  • Year and Date: 2011-07-13
  • Invited
• [Presentation] Pairing and recognition of homologous chromosomes in meiosis (2011)
  • Author(s): Hiraoka Y, Ding D-Q, Haraguchi T
  • Organizer: The 6th International Fission Yeast Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2011-06-29
  • Invited
• [Book] Live CLEM imaging: an application for yeast cells. In "Current microscopy contributions to advances in science and technology", vol.1; A. Mendez -Vilas (Ed.) (2012)
  • Author(s): Asakawa H, Hiraoka Y, Haraguchi T
  • Total Pages: 478-485
  • Publisher: Formatex Research Center
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/hiraoka/index.html