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2013 Fiscal Year Final Research Report

Development of useful microbial catalysts pioneering bio-based chemical industry

Research Project

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Project/Area Number 23248014
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionKyoto University

Principal Investigator

OGAWA Jun  京都大学, (連合)農学研究科(研究院), 教授 (70281102)

Co-Investigator(Kenkyū-buntansha) HIBI Makoto  京都大学, 大学院農学研究科, 助教 (30432347)
KISHINO Shigenobu  京都大学, 大学院農学研究科, 助教 (40432348)
ANDO Akinori  京都大学, 生理化学研究ユニット, 助教 (10537765)
Project Period (FY) 2011-04-01 – 2014-03-31
Keywords酵素合成 / 微生物変換 / ジオキシゲナーゼ / アシルCoA / NADH / FAD / 水酸化反応 / 飽和化反応
Research Abstract

Organic acids, alcohol, amino acids, sugars, and lipids generated by bio-refinery processes have different chemical structures from chemicals used in modern chemical industries. Chemicals used in petro based chemical industries, such as substrates for polymer synthesis, are highly reduced compounds; while biomass derived compounds are highly oxidized ones. In this research, bio-transformation technologies modifying biomass-derived oxidized compounds to highly reduced ones are investigated. The energy-supplying systems and electron follow systems supporting the reactions are also developed. For example, dicarboxylic acid, diol, olefin production from lipids were developed together with supporting cofactor regeneration systems and energy-providing systems.

  • Research Products

    (21 results)

All 2014 2013 2012 2011 Other

All Journal Article (17 results) (of which Peer Reviewed: 16 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Characteristics and biotechnology applications of aliphatic amino acid hydroxylases belonging to the Fe(II)/α-ketoglutarate-dependent dioxygenase superfamily2014

    • Author(s)
      Hibi, M., J. Ogawa
    • Journal Title

      Appl. Microbiol. Biotechnol.

      Volume: 98(9) Pages: 3869-3876

    • DOI

      10.1007/s00253-014-5620-z

    • Peer Reviewed
  • [Journal Article] Hydroxy fatty acid production by Pediococcus sp2013

    • Author(s)
      Takeuchi, M., S. Kishino, K. Tanabe, A. Hirata, S.B. Park, S. Shimizu, J. Ogawa
    • Journal Title

      Eur. J. Lipid Sci. Technol.

      Volume: 115(4) Pages: 386-393

    • DOI

      10.1002/ejlt.201200414

    • Peer Reviewed
  • [Journal Article] A novel l-isoleucine-4'-dioxygenase and L-isoleucine dihydroxylation cascade in Pantoea ananatis2013

    • Author(s)
      Smirnov, S.V., P.M. Sokolov, V.A. Kotlyarova, N.N. Samsonova, T. Kodera, M. Sugiyama, T. Torii, M. Hibi, S. Shimizu, K. Yokozeki, J. Ogawa
    • Journal Title

      MicrobiologyOpen

      Volume: 2(3) Pages: 471-481

    • DOI

      10.1002/mbo3.87

    • Peer Reviewed
  • [Journal Article] Crystal Structure of a Novel N-Substituted L-Amino Acid Dioxygenase from Burkholderia ambifaria AMMD2013

    • Author(s)
      Qin, H.M., T. Miyakawa, M.Z. Jia, A. Nakamura, J. Ohtsuka, Y.L. Xue, T. Kawashima, T. Kasahara, M. Hibi, J. Ogawa, M. Tanokura
    • Journal Title

      PLOS ONE

      Volume: 8(5) Pages: e63996

    • DOI

      10.1371/journal.pone.0063996

    • Peer Reviewed
  • [Journal Article] Polyunsaturated fatty acid saturation by gut lactic acid bacteria affecting host lipid composition2013

    • Author(s)
      Kishino, S., M. Takeuchi, S.B Park, A. Hirata, N. Kitamura, J. Kunisawa, H. Kiyono, R. Iwamoto, Y.Isobe, M. Arita, H. Arai, K. Ueda, J. Shima, S. Takahashi, K. Yokozeki, S. Shimizu, J. Ogawa
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 110(44) Pages: 17808-17813

    • DOI

      10.1073/pnas.1312937110/-/DCSupplemental

    • Peer Reviewed
  • [Journal Article] Characterization of a trifunctional fatty acid desaturase from oleaginous filamentous fungus Mortierella alpina 1S-4 using a yeast expression system2013

    • Author(s)
      Kikukawa, H., E. Sakuradani, S. Kishino, S.B. Park, A. Ando, J. Shima, M. Ochiai, S. Shimizu, J. Ogawa
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 116(6) Pages: 672-676

    • DOI

      10.1016/j.jbiosc.2013.05.023

    • Peer Reviewed
  • [Journal Article] 1-Leucine 5-hydroxylase of Nostoc punctiforme is a novel type of Fe(II)/α-ketoglutarate-dependent dioxygenase that is useful as a biocatalyst2013

    • Author(s)
      Hibi, M., T. Kawashima, P.M. Sokolov, S.V. Smirnov, T. Kodera, M. Sugiyama, S. Shimizu, K. Yokozeki, J. Ogawa
    • Journal Title

      Appl. Microbiol. Biotechnol.

      Volume: 97(6) Pages: 2467-2472

    • DOI

      10.1007/s00253-012-4136-7

    • Peer Reviewed
  • [Journal Article] Enzymatic synthesis of chiral amino acid sulfoxides by Fe(II)/α-ketoglutarate-dependent dioxygenase2013

    • Author(s)
      Hibi, M., T. Kawashima, H. Yajima, S.V. Smirnov, T. Kodera, M. Sugiyama, S. Shimizu, K. Yokozeki , J. Ogawa
    • Journal Title

      Tetrahedron : Asymmetry

      Volume: 24(17) Pages: 990-994

    • DOI

      10.1016/j.tetasy.2013.07.017

    • Peer Reviewed
  • [Journal Article] Polyunsaturated fatty acid production and transformation by Mortierelia alpina and anaerobic bacteria2012

    • Author(s)
      Ogawa, J., E. Sakuradani, S. Kishino, A. Ando, K. Yokozeki, S. Shimizu
    • Journal Title

      Eur. J. Lipid Sci. Technol.

      Volume: 114(10) Pages: 1107-1113

    • DOI

      10.1002/ejlt.201200069

    • Peer Reviewed
  • [Journal Article] Construction of microbial platform for an energy-requiring bioprocess : practical 2'-deoxyribonucleoside production involving a C-C coupling reaction with high energy substrates2012

    • Author(s)
      Horinouchi, N., T. Sakai, T. Kawano, S. Matsumoto, M. Sasaki, M. Hibi, J. Shima, S. Shimizu, J. Ogawa
    • Journal Title

      Microbial Cell Factories

      Volume: 11(82)

    • DOI

      10.1186/1475-2859-11-82

    • Peer Reviewed
  • [Journal Article] Oxidative pyrimidine metabolism in Rhodococcus erythropolis useful for valuable nucleoside synthesis : Discovery of a novel amidohydorolase, ureidomalonase2012

    • Author(s)
      Horinouchi, N., C.L. Soon, S. Shimizu, J. Ogawa
    • Journal Title

      Biocat. Agri. Biotechnol.

      Volume: 1(3) Pages: 264-266

    • DOI

      10.1016/j.bcab.2012.03.011

    • Peer Reviewed
  • [Journal Article] A novel Fe(II)/α-ketoglutarate-dependent dioxygenase from Burkholderia ambifaria has β-hydroxylating activity of N-succinyl L-leucine2012

    • Author(s)
      Hibi, M., T. Kawashima, T. Kasahara, P.M. Sokolov, S.V. Smirnov, T. Kodera, M. Sugiyama, S. Shimizu, K. Yokozeki, J. Ogawa
    • Journal Title

      Lett. Appl. Microbiol.

      Volume: 55(6) Pages: 414-419

    • DOI

      10.1111/j.1472-765X.2012.03308.x

    • Peer Reviewed
  • [Journal Article] Extracellular oxidases of Cerrena sp. complementarily functioning in artificial dye decolorization including laccase, manganese peroxidase, and novel versatile peroxidases2012

    • Author(s)
      Hibi, M., S. Hatahira, M. Nakatani, K. Yokozeki, S. Shimizu, J. Ogawa
    • Journal Title

      Biocat. Agri. Biotechnol.

      Volume: 1(3) Pages: 220-225

    • DOI

      10.1016/j.bcab.2012.03.003

    • Peer Reviewed
  • [Journal Article] β-Aryl-β-amino acid aminotransferase from Variovorax sp. JH2 is useful for enantioselective β-phenylalanineproduction2012

    • Author(s)
      Hibi, M., J. Mano, T. Hagishita, J. Shima, S. Shimizu, J. Ogawa
    • Journal Title

      Biocat. Agri. Biotechnol.

      Volume: 1(3) Pages: 253-258

    • DOI

      10.1016/j.bcab.2012.04.001

    • Peer Reviewed
  • [Journal Article] バイオプラ最前線-バイオマスからのモノマー生産を支援するバイオリピッドプラットフォームの構築-2012

    • Author(s)
      小川順,櫻谷英治,岸野重信,日比慎,安藤晃規
    • Journal Title

      バイオプラジャーナル

      Volume: 44 Pages: 13-19

  • [Journal Article] Novel multi-component enzyme machinery in lactic acid bacteria catalyzing C=C double bond migration useful for conjugated fatty acid synthesis2011

    • Author(s)
      Kishino, S., S.B. Park, M. Takeuchi, K. Yokozeki, S. Shimizu, J. Ogawa
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 416(1-2) Pages: 188-193

    • DOI

      10.1016/j.bbrc.2011.11.022

    • Peer Reviewed
  • [Journal Article] Linoleic acid isomerase in Lactobacillus plantarum AKU1009a proved to be a multi-component enzyme system requiring oxidoreduction cofactors2011

    • Author(s)
      Kishino, S., J. Ogawa, K. Yokozeki, S. Shimizu
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 75(2) Pages: 318-322

    • DOI

      10.1271/bbb.100699

    • Peer Reviewed
  • [Presentation] Application of microbial oxidizing enzymes for fine chemical synthesis and environmental Biotechnology2012

    • Author(s)
      Ogawa, J
    • Organizer
      The 12th Japan-China-Korea Joint Symposium on Enzyme Engineering
    • Place of Presentation
      Kanazawa, Japan
    • Year and Date
      20120528-31
  • [Presentation] Microbial Oxygenases as Catalysts for Fine Chemical Synthesis2012

    • Author(s)
      Ogawa, J
    • Organizer
      103rd AOCS Annual Meeting & Expo
    • Place of Presentation
      Long Beach, CA, USA
    • Year and Date
      20120429-0502
  • [Presentation] Development of Microbial Multi-component Enzyme Systems2011

    • Author(s)
      J. Ogawa
    • Organizer
      IUMS 2011 XIII International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      札幌
    • Year and Date
      2011-09-07
  • [Remarks]

    • URL

      http://www.hakko.kais.kyoto-u.ac.jp/

URL: 

Published: 2015-06-25  

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