Development of novel luciferin analogues for utilizing luciferases as new functional tools in vivo

2013 Fiscal Year Final Research Report

• Project/Area Number: 23249004
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: URANO Yasuteru 東京大学, 医学(系)研究科(研究院), 教授 (20292956)
• Project Period (FY): 2011-05-31 – 2014-03-31
• Keywords: 生物発光 / ルシフェラーゼ / ルシフェリン / 電子移動 / 細胞膜透過性 / 一酸化窒素 / 活性酸素 / プローブ

Outline of Final Research Achievements

Live imaging methods with activatable fluorescence probes, which show strong fluorescence only when the cells response to the target stimulants, are frequently used in modern biological experiments. However, the penetration of light is not so good for detecting cellular responses at deeper sites in living animals. This project aimed at the development of activatable bio-luminescence probes which show high bioluminescence only when the cells are active upon stimulation from outside. As a result, we succeeded in developing novel luciferin probes which emit quite long wavelength bioluminescence in near-infra red region, and activatable probes which emit strong fluorescence only when the cells response to some stimulants in vivo.

Free Research Field

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