Investigation of a novel technique for regeneration of elastic fibers with three-dimensional culture on a collagen sponge scaffold and the addition of latent TGF-beta binding protein 4

2015 Fiscal Year Final Research Report

• Project/Area Number: 23249078
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Kyoto University
• Principal Investigator: Suzuki Shigehiko 京都大学, 医学(系)研究科(研究院), 教授 (30187728)
• Co-Investigator(Kenkyū-buntansha): NAITOH Motoko 京都大学, 大学院・医学研究科, 講師 (30378723) ; KAWAI Katsuya 京都大学, 大学院・医学研究科, 准教授 (90273458)
• Co-Investigator(Renkei-kenkyūsha): NAKAMURA Tomoyuki 関西医科大学, 医学部, 教授 (20362527)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: 人工皮膚

Outline of Final Research Achievements

The objective of this study was to investigate the effects of latent TGF-β binding protein 4 (LTBP-4) on elastic fiber regeneration in three-dimensional cultures of human dermal fibroblasts (HDFs). Collagen sponges cross-linked at 120 °C and composed of small pores (25 μm on average) was favorable for elastic fiber regeneration by HDFs. Addition of LTBP-4, followed by culture for 21 days, accelerated elastic fiber accumulation within the scaffolds. Conditioned scaffolds containing either HDFs or LTBP-4-built mature elastic fibers were implanted between the dermis and the cutaneous muscle of mice. The combined use of HDFs and LTBP-4 resulted in thicker tissues containing elastic fibers. These results indicate that weakly cross-linked collagen sponges can be used as scaffolds for regenerating elastic fibers both in vitro and in vivo, and that the addition of LTBP-4 accelerates the deposition of both elastin and fibrillin-1, and increases cell proliferation.

Free Research Field

再生医療