2013 Fiscal Year Final Research Report
High sensitive fluorometric biosniffer based on UV-LED emission light and metabolizing enzyme
| Project/Area Number |
23300068
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Perception information processing/Intelligent robotics
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KUDO Hiroyuki 東京医科歯科大学, 生体材料工学研究所, 准教授 (70329118)
ARAKA WA Takahiro 東京医科歯科大学, 生体材料工学研究所, 講師 (50409637)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | UV-LED / 匂い / 高感度 / 選択性 / 代謝酵素 / 蛍光 / 揮発成分 |
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| Research Abstract |
A fluorometric biochemical gas sensor (biosniffer) for odor chemicals was developed by incorporating with an UV-LED emission light, a novel polymer PMEH (with low auto fluorescence) and metabolizing enzymes. The gas sensor immobilized formaldehyde dehydrogenase can successfully measure gaseous formaldehyde (lower detection limit: 750 ppt [parts per trillion]; human smell detection limit: 400 ppb [parts per billion]) with high gas selectivity. The sensor system was applicable for monitoring a different volatile chemical by changing it with other dehydrogenase enzyme.
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