2013 Fiscal Year Final Research Report
Development of the cryopreservation method for fish oocytes and ovaries
| Project/Area Number |
23300158
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Kochi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
EDASHIGE Keisuke 高知大学, 教育研究部総合科学系, 教授 (30175228)
KOSHIMOTO Chihiro 宮崎大学, フロンティア科学実験総合センター, 教授 (70295210)
MATSUKAWA Kazutsugu 高知大学, 教育研究部総合科学系, 准教授 (00532160)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 凍結保存 |
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| Research Abstract |
We tried to vitrify zebrafish oocytes at middle stage III and stage I successfully as a model of fish oocytes and ovaries, respectively. Oocytes at both stages were injected with cRNAs of water/cryoprotectant channels and anti-freeze proteins for exogenous expression of these proteins, and vitrified with a propylene glycol-based solution. Only oocytes expressing both proteins survived vitrification just after warming. However, the oocytes were dead after 1 h of culture. Further improvement is needed to succeed in the cryopreservation of fish oocytes and ovaries.
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[Journal Article] Increased metabolite levels of glycolysis and pentose phosphate pathway in rabbit atherosclerotic arteries and hypoxic macrophage2014
Author(s)
Atsushi Yamashita, Yan Zhao, Yunosuke Matsuura, Kazuaki Yamasaki, Sayaka Moriguchi-Goto, Chihiro Sugita, Takashi Iwakiri, Nozomi Okuyama, Chihiro Koshimoto, Keiichi Kawai, Nagara Tamaki, Songji Zhao, Yuji Kuge, Yujiro Asada
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Journal Title
PLos One
Volume: 9
Pages: e86426
DOI
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[Journal Article] Rapid movement of water and cryoprotectants in pig expanded blastocysts via channel processes : its relevance to their higher tolerance to cryopreservation2013
Author(s)
Bo Jin, Ryu-ichi Higashiyama, Yu-ichi Nakata, Jun-ichi Yonezawa, Shangdan Xu, Masashi Miyake, Sei-ichi Takahashi, Kazuhiro Kikuch, Ken-ichi Yazawa, Shuhei Mizobuchi, Saori Niimi, Mizuho Kitayama, Chihiro Koshimoto, Kazutsugu Matsukawa, Magosaburo Kasai, Keisuke Edashige
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Journal Title
Biology of Reproduction
Volume: 89
Pages: 1-12
DOI
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[Journal Article] Arterial ^<18>F-fluorodeoxyglucose uptake reflects thrombus formation and tissue factor expression via nuclear factor-κB in rabbit atherosclerotic lesions2013
Author(s)
Atsushi Yamashita, Yan Zhao, Songji Zhao, Yunosuke Matsuura, Chihiro Sugita, Takashi Iwakiri, Nozomi Okuyama, Kazuyo Ohe, Chihiro Koshimoto, Keiichi Kawai, Nagara Tamaki, Yuji Kuge, Yujiro Asada
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Journal Title
Circulation Journal
Volume: 77
Pages: 2626-2635
DOI
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[Presentation] ^<18>F-FDG uptake reflects thrombus formation and tissue factor expression via nuclear factor-κB in rabbit atherosclerotic lesions. American Heart Association2012
Author(s)
Atsushi Yamashita, Yan Zhao, Songji Zhao, Yunosuke Matsuura, Chihiro Sugita, Takashi Iwakiri, Kazuyo Ohe, Chihiro Koshimoto, Keiichi Kawai, Nagara Tamaki, Yuji Kuge, Yujiro Asada
Organizer
Scientific Session
Place of Presentation
JW Marriott Los Angeles at LA Live, Los Angeles, California
Year and Date
20121103-07
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[Presentation] The role of aquaporin 9 in the movement of DMSO and acetamide in mouse morulae2012
Author(s)
Keisuke Edashige, Shinsuke Seki, Hayato Arimura, Kan Matsuo, Yu-ichi Nakata, Bo Jin, Kazutsugu Matsukawa, Chihiro Koshimoto, Magosaburo Kasai
Organizer
SSR's 45th Annual Meeting of Society of the Study of Reprocution
Place of Presentation
The Alumni Hall at the Hetzel Union Building, The Pennsylvania State University, University Park, Pennsylvania
Year and Date
20120812-15
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