Physiomic analyses of cardiac hypertrophy and ventricular geometry of in cardiac regulation

2013 Fiscal Year Final Research Report

• Project/Area Number: 23300171
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kawasaki Medical School
• Principal Investigator: MOHRI Satoshi 川崎医科大学, 医学部, 教授 (00294413)
• Co-Investigator(Kenkyū-buntansha): HASHIMOTO Ken 川崎医科大学, 医学部, 講師 (80341080) ; UJIHARA Yoshihiro 川崎医科大学, 医学部, 助教 (80610021)
• Co-Investigator(Renkei-kenkyūsha): NARUSE Keiji 岡山大学, 医歯(薬)学総合研究科, 教授 (40252233)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 心機能 / 心肥大 / 心筋分裂 / 酸素環境 / 機械受容

Research Abstract

Cardiac pump function is determined by the quantity and quality of myocardium and ventricular geometry. We investigated the regulatory mechanism of the heart for the change in environment. First, we focused the effects of the change in oxygen tension on the cellular switching from division to hypertrophy during perinatal period. Fetal cardiomyocytes were harvested and cultured at different oxygen tension (low O2: 3%, high O2: 20%) . We firstly succeeded to visualize the division of cardiomyocytes using time-lapse motion picture under low O2 condition. Then, we found that cardiomyocytes stopped cell division under high O2 while they kept dividing under low O2 condition. We also performed micro-allay analysis to reveal the genes regulating cell cycle, which would be useful to make the adult cardiomyocytes to restart cell devision. As for the mechan-osensing in the heart, we show that TRPV2 cation channel is critical for the maintenance of cardiac structure and function.

Research Products

• [Journal Article] TRPV2 is critical for the maintenance of cardiac structure and function in mice (2014)
  • Author(s): Katanosaka Y, Iwasaki K, Ujihara Y, Takatsu S, Nishitsuji K, Kanagawa M, Sudo A, Toda T, Katanosaka K, Mohri S, Naruse K
  • Journal Title: Nat Commun Volume: 5 Pages: 3932
• [Presentation] TRPV2 is crucial for cardiac structure and function (2014)
  • Author(s): Katanosaka Y, Ujihara Y, Mohri S
  • Organizer: Gordon Research Conference
  • Place of Presentation: NH, USA (Colby-Sawyer College)
  • Year and Date: 2014-06-10
• [Presentation] TRPV2 maintains the structure and function of intercalated discs (2014)
  • Author(s): Mohri S, Ujihara Y, Katanosaka Y
  • Place of Presentation: NH, USA (Colby-Sawyer College)
  • Year and Date: 2014-06-10
• [Presentation] Isolated myocytes from TRPV2-deficient heart are fully functional (2014)
  • Author(s): Ujihara Y, Mohri S, Katanosaka Y
  • Organizer: Gordon Research Conference
  • Place of Presentation: NH, USA (Colby-Sawyer College)
  • Year and Date: 2014-06-10
• [Presentation] 甲状腺機能亢進症モデルマウスにおける心臓リモデリングの評価 (2013)
  • Author(s): 笹江友美, 橋本謙, 氏原嘉洋, 毛利聡
  • Organizer: 第65回日本生理学会大会中国四国地方会
  • Place of Presentation: 倉敷(川崎医科大学)
  • Year and Date: 2013-11-01
• [Presentation] T 間膜構造維持におけるNa^<+>/Ca^<2+>交換体の役割 (2013)
  • Author(s): 氏原嘉洋, 岩崎慶一朗, 高津理美, 西辻光希, 橋本謙, 成瀬恵治, 毛利聡, 片 野坂友紀
  • Organizer: 第65回日本生理学会大会中国四国地方会
  • Place of Presentation: 倉敷(川崎医科大学)
  • Year and Date: 2013-11-01