Study of genetic effects of atomic bomb radiation using high-density microarray

2014 Fiscal Year Final Research Report

• Project/Area Number: 23310042
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Radiation Effects Research Foundation
• Principal Investigator: KODAIRA Mieko 公益財団法人放射線影響研究所, 遺伝学部, 研究員 (60344412)
• Co-Investigator(Kenkyū-buntansha): ASAKAWA Jun-ichi (公財)放射線影響研究所, 遺伝学部, 主任研究員 (10359458) ; FURUKAWA Kyoji (公財)放射線影響研究所, 統計部, 副主任研究員 (00416421) ; NAKAMURA Nori (公財)放射線影響研究所, 遺伝学部, 顧問 (00010116)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: 原爆放射線の遺伝的影響 / 生殖細胞突然変異 / アレイCGH法による突然変異検索 / 突然変異の分子レベルの特徴

Outline of Final Research Achievements

We have analyzed a total of 127 mother-father-child trios using high-density microarrays. We identified about 300 copy number variants (CNV) per individual genome. Eleven CNVs observed in 11 children were not identified among the parents and were thought to be de novo germline mutations. Seven of the mutations were deletions; one was 140kb deletion but the remaining 6 were small deletions (5kb-30kb). Our model experiments on F1 mice derived from irradiated spermatogonia revealed that one deletion, which size is larger than 100kb, was induced among approximately 100 genomes per 1 Gy of radiation. The results of the current study on the offspring of the A-bomb survivors suggest that the radio-sensitivity of humans are not different from that of mice and the genetic risk of radiation maybe much smaller than currently estimated.

Free Research Field

分子遺伝学