2013 Fiscal Year Final Research Report
Two-dimensional alignment technique of biomolecules using microfluidic format
| Project/Area Number |
23310092
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
MIYAZAKI Masaya 独立行政法人産業技術総合研究所, 生産計測技術研究センター, 研究チーム長 (70344231)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Hiroshi 東海大学, 阿蘇教養教育センター, 准教授 (00466236)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | マイクロ化学システム / 表面 / 固定化 / 標識 |
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| Research Abstract |
In this study, we developed the multi-step enzymatic reaction technology using microchemical device to enable enzymatic reaction effectively to produce biologically active molecule. The two-dimensional alignment of molecule was enabled by using DNA as the templates. The template DNA molecules were immobilized within microchannel by using electorchemical methods or other techniques. Using this template, molecules can be immobilized and aligned on the DNA molecules. Furthermore, we developed microreactors for multi-step enzymatic reaction using these immobilized techniques. The microreactor showed possibility as an effective biologically active molecule processing device.
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