2013 Fiscal Year Final Research Report
Development of microchip for rapid detection of adipokines and early diagnosis of diabetes mellitus.
| Project/Area Number |
23310093
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
KATAOKA Masatoshi 独立行政法人産業技術総合研究所, 健康工学研究部門, 研究グループ長 (20224438)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | マイクロチップ基板 / サンドイッチELISA / 微細化インクジェット / IL-6 / TNF-α / アディポネクチン / 糖尿病 |
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| Research Abstract |
Interleukin-6 and TNF-a in the blood are frequently measured by conventional sandwich ELISA using 96 wells plate. However, the conventional assay is time-consuming, and is not suitable for rapid diagnosis. To overcome these drawbacks, we performed a sandwich ELISA on a microchip for simultaneous analysis. The microchip was made of cyclic olefin copolymer with 4 straight microchannels that were 300 um wide and 100 um deep. For the construction of the sandwich ELISA for IL-6 or TNF-a, we used a piezoelectric inkjet printing system for the deposition and fixation of the 1st antibody on the surface of the each microchannel. This microchip-based assay enabled us to determine simultaneously blood IL-6 and TNF-a with accuracy, satisfactory sensitivity, time saving ability, and low consumption of sample and reagents, and thus will be applicable to early diagnosis of diabetes mellitus.
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Research Products
(10 results)
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[Journal Article] Simultaneous immunoassay analysis of plasma IL-6 and TNF-α on a microchip2013
Author(s)
Abe K, Hashimoto Y, Yatsushiro S, Yamamura S, Bando M, Hiroshima Y, Kido J, Tanaka M, Shinohara Y, Ooie T, Baba Y, Kataoka M
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Journal Title
DOI
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[Journal Article] Singlet oxygen induced products of linoleates, 10-and 12(Z,E)-hydroxyoctadecadienoic acid (HODE), can be potential biomarkers for early detection of type 2 diabetes2013
Author(s)
Umeno A, Shichiri M, Ishida N, Hashimoto Y, Abe K, Kataoka M, Yoshino K, Hagihara Y, Aki N, Funaki M, Asada Y, Yoshida Y
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Journal Title
DOI
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[Journal Article] Analysis of proteins in human gingival crevicular fluid by mass spectrometry2012
Author(s)
Kido J, Bando M, Hiroshima Y, Iwasaka H, Yamada K, Ohgami N, Nambu T, Kataoka M, Yamamoto T, Shinohara Y, Sagawa I, Nagata T
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Journal Title
J Periodontal Res
Pages: 488-499
DOI
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