Development of microchip for rapid detection of adipokines and early diagnosis of diabetes mellitus.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23310093
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Microdevices/Nanodevices
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: KATAOKA Masatoshi 独立行政法人産業技術総合研究所, 健康工学研究部門, 研究グループ長 (20224438)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: マイクロチップ基板 / サンドイッチELISA / 微細化インクジェット / IL-6 / TNF-α / アディポネクチン / 糖尿病

Research Abstract

Interleukin-6 and TNF-a in the blood are frequently measured by conventional sandwich ELISA using 96 wells plate. However, the conventional assay is time-consuming, and is not suitable for rapid diagnosis. To overcome these drawbacks, we performed a sandwich ELISA on a microchip for simultaneous analysis. The microchip was made of cyclic olefin copolymer with 4 straight microchannels that were 300 um wide and 100 um deep. For the construction of the sandwich ELISA for IL-6 or TNF-a, we used a piezoelectric inkjet printing system for the deposition and fixation of the 1st antibody on the surface of the each microchannel. This microchip-based assay enabled us to determine simultaneously blood IL-6 and TNF-a with accuracy, satisfactory sensitivity, time saving ability, and low consumption of sample and reagents, and thus will be applicable to early diagnosis of diabetes mellitus.

Research Products

• [Journal Article] Simultaneous immunoassay analysis of plasma IL-6 and TNF-α on a microchip (2013)
  • Author(s): Abe K, Hashimoto Y, Yatsushiro S, Yamamura S, Bando M, Hiroshima Y, Kido J, Tanaka M, Shinohara Y, Ooie T, Baba Y, Kataoka M
  • Journal Title: PLoS One Pages: e53620
  • DOI: 10.1371/journal.pone.0053620
• [Journal Article] Singlet oxygen induced products of linoleates, 10-and 12(Z,E)-hydroxyoctadecadienoic acid (HODE), can be potential biomarkers for early detection of type 2 diabetes (2013)
  • Author(s): Umeno A, Shichiri M, Ishida N, Hashimoto Y, Abe K, Kataoka M, Yoshino K, Hagihara Y, Aki N, Funaki M, Asada Y, Yoshida Y
  • Journal Title: PLoS One Pages: E63542
  • DOI: 10.1371/journal.pone.0063542
• [Journal Article] Mechanism of interleukin-1α transcriptional regulator of S100A9 in a human epidermal keratinocyte cell line (2013)
  • Author(s): Bando M, Zou X, Hiroshima Y, Kataoka M, Ross KF, Shinohara Y, Nagata T, Herzberg MC, Kido J
  • Journal Title: Biochem Biophys Acta Pages: 954-962
  • DOI: 10.1016/j.bbagrm.2013.03.010
• [Journal Article] Development of microchip for the analysis of biomarkers in blood (2012)
  • Author(s): Kataoka M, Abe K, Hashimoto Y, Yamamura S, Yatsushiro S
  • Journal Title: Rinsho Byori Pages: 1094-1100
  • URL: http://www.jslm.org/books/journal/60_11.pdf
• [Journal Article] Detection of miRNA in cell cultures by using microchip electrophoresis with a fluorescence-labeled riboprobe (2012)
  • Author(s): Yamamura S, Yatsushiro S, Yamaguchi Y, Abe K, Shinohara Y, Kataoka M
  • Journal Title: Sensors Pages: 7576-7586
  • DOI: 10.3390/s120607576
• [Journal Article] Determination of calprotectin in gingival crevicular fluid by immunoassay on a microchip (2012)
  • Author(s): Kido J, Abe K, Yatsushiro S, Bando M, Hiroshima Y, Nagata T, Ooie T, Tanaka M, Kataoka M
  • Journal Title: Clin Biochem Pages: 1239-1244
  • DOI: 10.1016/j.clinbiochem.2012.05.009
• [Journal Article] Accurate detection of carcinoma cells by use of a cell microarray chip (2012)
  • Author(s): Yamamura S, Yatsushiro S, Yamaguchi Y, Abe K, Shinohara Y, Tamiya E, Baba Y, Kataoka M
  • Journal Title: PLoS One Pages: e32370
  • DOI: 10.1371/journal.pone.0032370
• [Journal Article] Resistin in gingival crevicular fluid and induction of resistin release by Porphyromonas gingivalis lipopolysaccharide in human neutrophils (2012)
  • Author(s): Hiroshima Y, Bando M, Inagaki Y, Mihara C, Kataoka M, Murata H, Shinohara Y, Nagata T, Kido J
  • Journal Title: J Periodontal Res Pages: 554-652
  • DOI: 10.1111/j.1600-0765.2011.01466.x
• [Journal Article] Analysis of proteins in human gingival crevicular fluid by mass spectrometry (2012)
  • Author(s): Kido J, Bando M, Hiroshima Y, Iwasaka H, Yamada K, Ohgami N, Nambu T, Kataoka M, Yamamoto T, Shinohara Y, Sagawa I, Nagata T
  • Journal Title: J Periodontal Res Pages: 488-499
  • DOI: 10.1111/j.1600-0765.2011.01458.x
• [Presentation] Quantitative, rapid and high sensitive analysis of adipokine in blood by immunoassay on microchip (2014)
  • Author(s): Hashimoto Y, Abe K, Obana E, Kataoka M
  • Organizer: Advanced in Biodetection & Biosensors
  • Place of Presentation: Berlin, Germany
  • Year and Date: 20140310-11