2013 Fiscal Year Final Research Report
Roles of type II DNA topoisomerase in the interaction of distant genomic sites
| Project/Area Number |
23310133
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Okayama University |
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Principal Investigator |
TSUTSUI KEN 岡山大学, 医歯(薬)学総合研究科, 名誉教授 (70108158)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAJI Mary 岡山大学, 大学院・医歯薬学総合研究科, 助教 (50349255)
HOSOYA Osamu 岡山大学, 大学院・医歯薬学総合研究科, 助教 (90304310)
SANO Kuniaki 岡山大学, 大学院・医歯薬学総合研究科, 助教 (00294405)
TSUTSUI Kimiko 岡山大学, 大学院・医歯薬学総合研究科, 助教 (70144748)
KUWANO Ryozo 新潟大学, 脳研究所, 教授 (20111734)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 遺伝子 / ゲノム / 酵素 / 発現制御 / 発生・分化 |
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| Research Abstract |
We analyzed the characteristic gene regulation mechanism in terminally differentiating neuronal cells by identifying the genomic region targeted by DNA topoisomerase IIbeta (topo IIb) and its partner protein SP120/hnRNP U through massive sequencing on new generation sequencers. Results are summarized as follows: 1) Topo IIb regulates the transcription of groups of genes in both directions (up- and down-regulation). 2) Genes controlled by topo IIb are positioned non-randomly on the genome. 3) Analysis of the topo IIb action sites (toposites) suggested that the toposite enriched in intergenic regions (termed Ts3) has an important role in the gene expression of terminal differentiation. 4) The SP120-binding sites overlapping with Ts3 toposites appear to be the target of topo IIb-SP120 complex, which was also demonstrated by cytological methods as nuclear colocalization spots. 5) Ts3 toposites represent the topo IIb-mediated distant interaction sites involved.
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Research Products
(13 results)
