2013 Fiscal Year Final Research Report
Lineage potential regulated by high order chromatin structure
| Project/Area Number |
23310134
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Seiji 九州大学, 医学(系)研究科(研究院), 准教授 (30448435)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | クロマチン / 骨格筋分化 / 次世代シークエンサー / ジーンクラスタリング |
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| Research Abstract |
Lineage potential is triggered by lineage-specific transcription factor expression in association with structural chromatin changes. Histone H3.3 variant is a critical chromatin component that regulates lineage potential, but the function of each H3 variant remains unclear. Here, we found that forced incorporation of H3.1 (canonical H3 variant) into lineage-specific genes diminished trimethylation on H3K4 (H3K4me3) and increased trimethylation on H3K27 (H3K27me3), resulting in loss of lineage potential. In mouse embryos, bivalent modifications of H3K4me3 and H3K27me3 were equivalently formed on the H3.3-incorporated region before embryonic skeletal muscle differentiation at myogenic loci. These results suggest that lineage potential is established through selective H3.3 incorporation into chromatin and is defined by a quantitative balance among histone modifications.
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