2013 Fiscal Year Final Research Report
Development of high throughput image cytometry for microalgal screening toward oil production
| Project/Area Number |
23360363
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
TANAKA Tsuyoshi 東京農工大学, 工学(系)研究科(研究院), 准教授 (20345333)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUDA Yorikane 東京農工大学, 大学院・工学府, 特任助教 (40435855)
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| Project Period (FY) |
2011-11-18 – 2014-03-31
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| Keywords | 高速イメージサイトメトリー / 微細藻類 / スクリーニング / バイオ燃料 |
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| Research Abstract |
High throughput image cytometry was developed for screening of microalgae with high oil production. The microcavity-culture device created large scale cell patterning, and then time-lapse imaging was performed to observe cell growth and oil accumulation. Additionally, colony formation monitoring system was also developed using a CMOS image sensor. It improved observation throughput, which allowed us to overcome current limitation of the microalgal screening. The image cytometry system developed in this study is expected to be used to explore promising microalgal producers of biofuel, as well as other variable materials including carotenoid, glycosphingolipid and polyunsaturated lipid.
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