Functions of RECQL1 and RECQL5 in the maintenance of genome stability

2013 Fiscal Year Final Research Report

• Project/Area Number: 23370065
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Musashino University
• Principal Investigator: ENOMOTO Takemi 武蔵野大学, 薬学研究所, 教授 (80107383)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: ゲノム安定維持機構 / 発がん抑制 / 遺伝子組換え / RecQヘリカーゼ / RECQL5

Research Abstract

In this study, I mainly focused on the function of RECQL5. Analyses using RECQL5 gene knockout (KO) cells revealed that RECQL5 is involved in DNA interstrand crosslink (ICL) repair. The results obtained in this study suggest that RECQL5 functions down stream of BRCA2, after formation of Rad51-ssDNA filament probably disrupting disused filaments. Furthermore, the frequency and variation of CDDP-induced gene conversion at the immunoglobulin locus were increased in RECQL5 KOcells. These results suggest that RECQL5 plays a role in regulating the incidence and quality of ICL-induced recombination.

Research Products

• [Journal Article] Tumor suppressor RecQL5 controls recombination induced by DNA crosslinking agents (2014)
  • Author(s): Hosono, Y., Abe, T., Ishiai, M., Islam M. N., Arakawa H., Wang, W., Takeda, S., Ishii, Y., Takata, M., Seki, M., and Enomoto, T
  • Journal Title: Biochim. Biophys. Acta 1843 Pages: 1002-1012
  • DOI: 10.1016/j.bbamcr.2014.01.005
• [Journal Article] Tipin functions in the protection against topoisomerase I inhibitor (2014)
  • Author(s): Hosono, Y., Abe, T., Higuchi, M., Kajii, K., Sakuraba, S., Tada, S., Enomoto, T., and Seki, M
  • Journal Title: J. Biol. Chem Volume: 289 (16) Pages: 11374-11384
  • DOI: 10.1074/jbc.M113.531707
• [Journal Article] Roles of common subunits within distinct multisubunit complexes (2014)
  • Author(s): Nakabayashi, Y., Kawashima, S., Enomoto, T., Seki, M., and Horikoshi, T.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 111 Pages: 699-704
  • DOI: 10.1073/pnas.1316433111
• [Journal Article] Protective roles of ascorbic acid in oxidative stress induced by depletion of superoxide dismutase in vertebrate cells (2013)
  • Author(s): Tamari, Y., Nawata, H., Inoue, E., Yoshimura, A., Yoshii, H., Kashino, G., Seki, M., Enomoto, T., Watanabe, M., and Tano, K
  • Journal Title: Free Rad. Res Volume: 47 Pages: 1-7
  • DOI: 10.3109/10715762.2012.734916
• [Journal Article] Rmi1 functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1 (2012)
  • Author(s): Lai, M.S., Seki, M., Tada, S., and Enomoto, T
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 427 Pages: 682-686
  • DOI: 10.1016/j.bbrc.2012.09.12
• [Journal Article] NIH3T3 cells overexpressing CD98 heavy chain resist early G1 arrest and apoptosis induced by serum starvation (2012)
  • Author(s): Hara, K., Ueda, S., Ohno, Y., Tanaka, T., Yagi, H., Okazaki, S., Kawahara, R., Masayuki, T., Enomoto, T., Hashimoto, Y., Masuko, K., and Masuko, T
  • Journal Title: Cancer Sci Volume: 103
  • DOI: 10.1111/j.1349-7006.2012.02304.x1460-1466.
• [Journal Article] An FD-LC-MS/MS proteomic strategy for revealing cellular protein networks : A conditional superoxide dismutase 1 knockout cells (2012)
  • Author(s): Ichibangase, T., Sugawara, Y., Yamabe, A., Koshiyama, A., Yoshimura, A., Enomoto, T., and Imai, K
  • Journal Title: PLOS ONE Volume: 7 Pages: e45483
  • DOI: 10.1371/journal.pone.0045483
• [Journal Article] WRNIP1 accumulates at laser light irradiated sites rapidly via its ubiquitin-binding zinc finger domain and independently from its ATPase domain (2012)
  • Author(s): Nomura, H., Yoshimura, A., Edo, T., Tada, S., Seki, M., and Enomoto, T
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 417 Pages: 1145-1150
  • DOI: 10.1016/j.bbrc.2011.12.080
• [Journal Article] Nucleosome surface containing nucleosomal DNA entry/exit site regulates H3-K36me3 via association with RNA polymerase II and Set2 (2012)
  • Author(s): Endo, H., Nakabayashi, Y., Kawashima, S., Enomoto, T., Seki, M., and Horikoshi, M
  • Journal Title: Genes Cells Volume: 17 Pages: 65-81
  • DOI: 10.1111/j.1365-2443.2011.01573.x
• [Journal Article] Embryonic lethality in mice lacking mismatch-specific thymine DNA glycosylase is partially prevented by DOPS a precursor of noradrenarine (2012)
  • Author(s): Saito, Y., Ono, T., Takeda, N., Nohmi, T., Seki, M., Enomoto, T., Noda, T., and Uehara Y
  • Journal Title: Tohoku J. Exp. Med Volume: 226 Pages: 75-83
• [Journal Article] Functional relationship between Claspin and Rad17. Biochem (2011)
  • Author(s): Yoshimura, A., Akita, M., Hosono, Y., Abe, T., Kobayashi, M., Yamamoto, K., Tada, S., Seki, M., and Enomoto, T
  • Journal Title: Biophys. Res. Commun Volume: 414 Pages: 298-303
  • DOI: 10.1016/j.bbrc.2011.09.037
• [Journal Article] Roles of histone chaperone CIA/Asf1 in nascent DNA elongation during nucleosome replication (2011)
  • Author(s): Ishikawa, K., Ohsumi, T., Tada, S., Natsume, R., Kundu, L.R., Nozaki, N., Senda, T., Enomoto, T., Horikoshi, M., and Seki, M
  • Journal Title: Genes Cells 16 Volume: 16 Pages: 1050-1062
  • DOI: 10.1111/j.1365-2443.2011.01549.x
• [Journal Article] The histone chaperone FACT maintains normal replication rates (2011)
  • Author(s): Abe, T., Sugimura, K., Hosono, Y., Takami, Y., Akita, M., Yoshimura, A., Tada, S., Nakayama, T., Murofushi, H., Okumura, K., Takeda, S., Horikoshi, M., Seki, M., and Enomoto, T
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 30504-30512
  • DOI: 10.1074/jbc.M111.264721
• [Journal Article] Global analysis of core histones reveals nucleosomal surfaces required for chromosome bi-orientation (2011)
  • Author(s): Kawashima, S., Nakabayashi, Y., Matsubara, K., Sano, N., Enomoto, T., Tanaka, K., Seki, M., and Horikoshi, M
  • Journal Title: EMBO J Volume: 30 Pages: 3353-3367
  • DOI: 10.1038/emboj.2011.241
• [Journal Article] The role of SNM1 family nucleases in etoposide-induced apoptosis (2011)
  • Author(s): Hosono, Y., Abe, T., Ishiai, M., Takata, M., and Enomoto, T.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 410 Pages: 568-573
  • DOI: 10.1016/j.bbrc.2011.06.027
• [Journal Article] Werner interacting protein 1 promotes binding ofWerner protein to template-primer DNA (2011)
  • Author(s): Kanamori, M., Seki, M., Yoshimura, A., Tsurimoto, T., Tada, S., and Enomoto, T.
  • Journal Title: Biol. Pharm. Bull Volume: 34 Pages: 1314-1318
• [Journal Article] Biphasic chromatin binding of FACT during DNA replication (2011)
  • Author(s): Kundu, L. R., Seki, M., Watanabe, N., Murofusi H,, Furukohri, A., Waga, S., Score A. J., Blow, J. J., Horikoshi, M., Enomoto, T., and Tada, S
  • Journal Title: Biochim. Biophys. Acta Volume: 1813 Pages: 1129-1136
  • DOI: 10.1016/j.bbamcr.2011.01.002
• [Journal Article] The N-terminal region of RECQL4 lacking the helicase domain is both essential and sufficient for the viability of vertebrate cells (2011)
  • Author(s): Abe, T., Yoshimura, A., Hosono, Y., Tada, S., Seki, M., and Enomoto, T.
  • Journal Title: Biochim. Biophys. Acta Volume: 1813 Pages: 473-479
  • DOI: 10.1016/j.bbamcr.2011.01.001
• [Presentation] WRNIP1 regulates DNA polymerase eta in DNA damage tolerance (2013)
  • Author(s): 吉村明, 薗田覚, 関政幸, 榎本武美
  • Organizer: 第86回日本生化学大会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2013-09-13
• [Presentation] RecQヘリカーゼのDNAクロスリンク修復における役割 (2012)
  • Author(s): 細野嘉史, 関政幸, 阿部拓也, 石合正道, 武田俊一, 石井裕, 高田穣, 榎本武美
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-12-11
• [Presentation] Function of RecQL5 helicase in interstrand crosslink repair (2012)
  • Author(s): Yoshifumi Hosono, Masayuki Seki, Takuya Abe, Masamichi Ishiai, Shunichi Takeda, Ishii Yutaka, Minoru Takata, Takemi Enomoto
  • Organizer: The 8th 3R Symposium
  • Place of Presentation: 淡路夢舞台(淡路市)
  • Year and Date: 2012-11-26
• [Presentation] Analysis of the functional relationship between WRNIP1 and DNA polymerase eta in the cell (2011)
  • Author(s): 吉村明, 関政幸, 榎本武美
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-15
• [Presentation] DNAヘリカーゼRecQL5の相同組換え機構における機能の解析 (2011)
  • Author(s): 細野嘉史, 阿部拓也, 関政幸, 榎本武美
  • Organizer: DNAヘリカーゼRecQL5の相同組換え機構における機能の解析
  • Place of Presentation: サンピア福岡(福津市)
  • Year and Date: 2011-10-25